Methods for expansion and analysis of cultured hematopoietic stem cells
AssigneeWhitehead Institute for Biomedical Research
InventorsChengcheng Zhang, Harvey F. Lodish
Life of this patent
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Abstract
Methods and kits for propagating hematopoietic stem cells are provided. The methods comprise culturing cells in medium comprising one or more angiopoietin-like proteins, under conditions sufficient for expansion of HSCs. Angiopoietin-like proteins include angiopoietin-like protein 2, angiopoietin-like protein 3, angiopoietin-like protein 4, angiopoietin-like protein 5, angiopoietin-like protein 7, and microfibrillar-associated glycoprotein (Mfap4). Methods for identifying hematopoietic stem cells are provided and isolated hematopoietic stem cells are also provided.
Claims39
A method of propagating hematopoietic stem cells (HSCs) in vitro comprising culturing a population of cells comprising HSCs in a culture medium comprising an angiopoietin-like protein and stem cell factor (SCF).
A method of propagating hematopoietic stem cells comprising culturing at least one primary hematopoietic stem cell for at least five days in a serum free culture medium comprising (i) an angiopoietin-like protein selected from the group consisting of angiopoietin-like protein 2, angiopoietin-like protein 3, angiopoietin-like protein 4, angiopoietin-like protein 5, angiopoietin-like protein 7, and Mfap4 and (ii) stem cell factor (SCF).
A method of propagating hematopoietic stem cells comprising culturing at least one human cell for at least five days in a serum free culture medium comprising angiopoietin-like protein 5 and stem cell factor (SCF), wherein at least one of the human cells is a hematopoietic stem cell.
A method of administering hematopoietic stem cells to an individual comprising:
- a) obtaining hematopoietic stem cells from the individual or a donor;
- b) propagating the hematopoietic stem cells in a culture medium comprising an angiopoietin-like protein and stem cell factor (SCF), and
- c) transplanting the cultured cells into the individual.
Dependencies are inferred from the claim text.