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US 7,807,459 B2Granted
EphA4-positive human adult pancreatic endocrine progenitor cells
AssigneeReneuron Limited
InventorsWen-Ghih Tsang, Yanping Wang, Jinghua Tang
Life of this patent
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Abstract
The invention relates to the discovery of a selective cell surface marker that permits the selection of a unique subset of pancreatic stem cells having a high propensity to differentiate into insulin-producing cells or into insulin-producing cell aggregates.
Claims37
A method of obtaining a culture of propagating pancreatic cells comprising:
- (a) isolating pancreatic cells from a pancreas;
- (b) contacting the pancreatic cells with an EphA4 binding reagent;
- (c) selecting pancreatic cells that specifically bind to the EphA4 binding reagent;
- and
- (d) separating the selected pancreatic cells from pancreatic cells that do not bind the EphA4 binding reagent to obtain a culture of propagating EphA4+ pancreatic cells, wherein the EphA4+ pancreatic cells have the capacity to differentiate into insulin producing cells.
2 more elements
A method of producing an aggregate of insulin-producing pancreatic cells comprising the steps of:
- (a) isolating pancreatic cells from a pancreas;
- (b) contacting the pancreatic cells with an EphA4 binding reagent;
- (c) selecting pancreatic cells that specifically bind to the EphA4 binding reagent;
- (d) separating the selected pancreatic cells from pancreatic cells that do not bind the EphA4 binding reagent to obtain a culture of propagating EphA4+ pancreatic cells;
- and
- (e) differentiating the propagating EphA4+ pancreatic cell culture into an aggregate of insulin producing pancreatic cells.
3 more elements
A method of providing insulin to a mammal in need thereof, the method comprising the steps of:
- (a) isolating pancreatic cells from a pancreas;
- (b) contacting the pancreatic cells with an EphA4 binding reagent;
- (c) selecting pancreatic cells that specifically bind to the EphA4 binding reagent;
- (d) separating the selected pancreatic cells from pancreatic cells that do not bind the EphA4 binding reagent to obtain a culture of propagating EphA4+ pancreatic cells;
- and
- (e) implanting into the mammal the propagating pancreatic cells in an amount sufficient to produce a measurable amount of insulin in the mammal.
3 more elements
Dependencies are inferred from the claim text.