Preparation of purified exosomes comprising HCV RNA
AssigneeNovartis AG
InventorsSergio Abrignani, Piero Pileri
Life of this patent
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Abstract
The invention relates to a method for isolation of hepatitis C virus. The method comprises the separation of particles termed exosomes from the blood plasma of an individual infected with hepatitis C virus (HCV) and the extraction or RNA from these exosome particles.
Claims14
A purified preparation of exosomes that comprise hepatitis C virus (HCV) RNA prepared by a method comprising separating exosome particles from the supernatant of a human cell culture infected with HCV using iterative centrifugation.
A purified preparation of exosomes that comprise hepatitis C virus (HCV) RNA prepared by a method comprising separating exosome particles from the supernatant of a human cell culture infected with HCV, wherein said exosome particles are prepared from the cell culture supernatant by incubating the cell culture supernatant with beads coated with antibody.
A preparation of exosomes that comprise hepatitis C virus (HCV) RNA prepared by a method comprising:
- (a) providing plasma from a subject infected with HCV;
- (b) subjecting the plasma to differential centrifugation to produce an exosome-enriched supernatant;
- and
- (c) incubating the supernatant with beads coated with an anti-CD81 or anti-CD82 antibody to isolate exosome particles.
A preparation of exosomes that comprise hepatitis C virus (HCV) RNA prepared by a method comprising
- (a) providing plasma from a human infected with HCV;
- (b) subjecting the plasma to differential centrifugation to produce an exosome-enriched supernatant;
- and
- (c) incubating the supernatant with magnetic beads coated with an anti-CD81 or anti-CD82 antibody to isolate exosome particles.
Dependencies are inferred from the claim text.