Reagent for fluorimetric determination of analytes
AssigneeRoche Diagnostics Operations, Inc.
InventorsCarina Horn, Hans-Peter Josel, Juergen Spinke, Rupert Herrmann, Dieter Heindl
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Abstract
A method and reagent for detecting the presence or amount of an analyte by a redox reaction and a fluorimetric determination, is disclosed. The reagent comprises a compound of the general formula Q-F as a redox indicator, wherein Q is a quencher group and F is a fluorophore group. The quencher group Q or/and the fluorophore group F can be reduced or oxidized, and the fluorescence can change depending on the reduction or oxidation.
Claims12
A reagent for detecting the presence or amount of an analyte by a redox reaction and a fluorimetric determination comprising:
- a compound of the general formula Q-F as a redox indicator, wherein Q is a quencher group and F is a fluorophore group, the quencher group Q can be reduced or oxidized by said redox reaction, and the fluorescence activity of said Q-F compound can change depending on if said quencher group of said Q-F compound is present in a reduced or oxidized state;
- and
- an enzyme for reducing or oxidizing the analyte and optionally a coenzyme.
Dependencies are inferred from the claim text.