Nuclear magnetic resonance measuring method for solid samples
AssigneeOtsuka Pharmaceutical Co., Ltd.
InventorsIwao Miura, Masahiro Miyake
Life of this patent
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Abstract
In the IR (Inversion Recovery) method, to observe a phenomenon in which a nuclear spin returns to a steady state, a 90-degree pulse is irradiated after a lapse of time τ after finishing applying a 180-degree pulse. An FID signal starts being processed after a lapse of reception delay time Dd after finishing applying the 90-degree pulse. Among the FID signals from protons in a plurality of environments in a solid sample, an FID signal high in time-dependent attenuation rate almost disappears during the lapse of reception delay time Dd. Therefore, an FID signal of a proton in an environment intended to be measured can be easily selected and extracted from FID signals of other protons.
Claims12
A nuclear magnetic resonance measuring method for solid samples, the method comprising the steps of:
- exciting nuclear spins of a solid sample placed in a static magnetic field by causing an exciting pulse to flow through a coil;
- receiving an FID signal (Free Induction Decay signal) from the excited nuclear spins after applying a reading pulse after a lapse of time t from an end of application of the exciting pulse;
- and obtaining a DIR (Delayed Inversion Recovery)-PMR (Proton Magnetic Resonance) spectrum by subjecting the FID signal to a frequency conversion process,
- wherein the FID signal frequency conversion process starts after a lapse of reception delay time after an end of application of the reading pulses, and
- wherein water being present in and around the solid sample is caught by a water molecule trapping mechanism that traps water molecules through atmosphere during measurement.
A nuclear magnetic resonance measuring method for solid samples, the method comprising the steps of:
- exciting nuclear spins of a solid sample placed in a static magnetic field by causing an exciting pulse to flow through a coil;
- receiving an FID signal (Free Induction Decay signal) from the excited nuclear spins after applying a reading pulse after a lapse of time t from an end of application of the exciting pulse;
- and obtaining a DIR (Delayed Inversion Recovery)-PMR (Proton Magnetic Resonance) spectrum by subjecting the FID signal to a frequency conversion process,
- further comprising the steps of:
- acquiring a plurality of FID signals while changing the period of time t,
- calculating DIR-PMR spectra based on these FID signals wherein each of the DIR-PMR spectrum is applied by a digital smoothing filter to remove noise,
- obtaining a longitudinal relaxation curve by plotting spectrum strength with respect to time t in a specific frequency of the DIR-PMR spectrum, and
- making a regression analysis while regarding the longitudinal relaxation curve as a total sum of a plurality of longitudinal relaxation curves differing in longitudinal relaxation time, thereby estimating a constituent ratio of constituent substances of the solid sample.
A nuclear magnetic resonance measuring method for solid samples, the method comprising the steps of:
- exciting nuclear spins of a solid sample placed in a static magnetic field by causing an exciting pulse to flow through a coil;
- receiving an FID signal (Free Induction Decay signal) from the excited nuclear spins after applying a reading pulse after a lapse of time t from an end of application of the exciting pulse;
- and obtaining a DIR (Delayed Iverson Recovery)-PMR (Proton Magnetic Resonance) spectrum by subjecting the FID signal to a frequency conversion process, further comprising the steps of:
- acquiring a plurality of FID signals while changing the period of time t,
- pre-applying a smoothing process to the FID signals along a time axis,
- calculating DIR-PMR spectra based on these FID signals,
- obtaining a longitudinal relaxation curve by plotting spectrum strength with respect to time tin a specific frequency of the DIR-PMR spectrum, and
- making a regression analysis while regarding the longitudinal relaxation curve as a total sum of a plurality of longitudinal relaxation curves differing in longitudinal relaxation time, thereby estimating a constituent ratio of constituent substances of the solid sample.
Dependencies are inferred from the claim text.