Methods for detecting dihydrotestosterone by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsAmit Ghoshal, Nigel J. Clarke, Richard Reitz
Life of this patent
Estimating the term…
Abstract
Provided are methods for determining the amount of dihydrotestosterone (DHT) in a sample using mass spectrometry. The methods generally involve ionizing DHT in a sample and detecting and quantifying the amount of the ion to determine the amount of DHT in the sample.
Claims32
A method for determining the amount of underivatized dihydrotestosterone (DHT) in a body fluid sample, said method comprising:
- a. ionizing underivatized DHT, purified by solid phase extraction (SPE) from the body fluid sample under conditions suitable to produce one or more ions detectable by mass spectrometry;
- wherein said ions comprise one or more ions selected from the group consisting of ions with a mass/charge ratio of 291.10±0.50, 255.20±0.50, and 79.20±0.50;
- and
- b. determining the amount of one or more ions by mass spectrometry, and using the amount determined to calculate the amount of underivatized DHT in the body fluid sample.
A method for determining the amount of underivatized dihydrotestosterone (DHT) in a test sample by mass spectrometry, said method comprising:
- a. ionizing underivatized DHT, purified from the test sample by high turbulence liquid chromatography (HTLC), under conditions suitable to produce one or more ions detectable by mass spectrometry;
- and
- b. determining the amount of one or more ions by mass spectrometry, and using the amount determined to calculate the amount of underivatized DHT in the body fluid sample.
A method for determining the amount of underivatized dihydrotestosterone (DHT) in a body fluid sample by tandem mass spectrometry said method comprising:
- a. generating a precursor ion of underivatized DHT, purified from a body fluid sample by high turbulence liquid chromatography (HTLC), having a mass/charge ratio of 291.10±0.50;
- b. generating one or more fragment ions of said precursor ion, wherein at least one of said one or more fragment ions comprise a fragment ion selected from the group of ions having a mass/charge ratio of 255.20±0.50 and 79.20±0.50;
- and
- c. determining the amount of one or more of said ions generated in step (b) or (c) or both, and using the amount determined to calculate the amount of underivatized DHT in said test sample.
Dependencies are inferred from the claim text.