Methods and compositions for modifying flower development
AssigneePioneer Hi-Bred International, Inc.
InventorsWesley B Bruce, Suling Zhao
Life of this patent
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Abstract
The present invention provided methods and compositions for modifying flower development and reproductive development in plants. The methods involve transforming a plant with a polynucleotide construct comprising a promoter operably lined to polynucleotide molecule encoding a short vegetative phase-like transcription factor. The compositions include transformed plants, plant cells, seeds, and expression cassettes comprising a polynucleotide molecule encoding a short vegetative phase-like transcription factor or fragment or variant thereof.
Claims50
A method for modifying flower development in a plant, said method comprising transforming a plant with a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that is expressed in a plant cell, wherein the polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:1, 3 or 4;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:2;
- (c) a nucleotide sequence comprising at least 95% sequence identity to SEQ ID NO:1 or 3, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (d) a nucleotide sequence encoding an amino acid sequence having at least 95% sequence identity to SEQ ID NO:2, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- and
- (e) a nucleotide sequence that is the complement of the nucleotide sequence of (a), (b), (c), or (d);
- and selecting a transformed plant having modified flower development when compared to an untransformed plant.
A method for modifying reproductive development in a plant, said method comprising transforming a plant with a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that is expressed in a plant cell, wherein the polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:1, 3 or 4;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:2;
- (c) a nucleotide sequence comprising at least 95% sequence identity to SEQ ID NO:1 or 3, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (d) a nucleotide sequence encoding an amino acid sequence having at least 95% sequence identity to SEQ ID NO:2, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- and
- (e) a nucleotide sequence that is the complement of the nucleotide sequence of (a), (b), (c), or (d);
- and selecting a transformed plant having modified reproductive development when compared to an untransformed plant.
A method for increasing the grain yield of a grain plant, said method comprising transforming a plant with a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that is expressed in a plant cell, wherein the polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:1, 3 or 4;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:2;
- (c) a nucleotide sequence comprising at least 95% sequence identity to SEQ ID NO:1 or 3, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- (d) a nucleotide sequence encoding an amino acid sequence having at least 95% sequence identity to SEQ ID NO:2, wherein said polynucleotide molecule encodes a polypeptide having ZmSVP activity;
- and
- (e) a nucleotide sequence that is the complement of the nucleotide sequence of (a), (b), (c), or (d);
- and selecting a transformed plant having increased grain yield when compared to an untransformed plant.
A transformed plant comprising a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that drives expression in the plant, wherein said polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- and
- (c) a nucleotide sequence that is the complement of the nucleotide sequence of (a) (b).
A progeny plant of a transformed plant, said transformed plant comprising a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that drives expression in the plant, wherein said polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- and
- (c) a nucleotide sequence that is the complement of the nucleotide sequence of (a) or (b);
- wherein the progeny plant comprises the polynucleotide construct.
A transformed plant cell comprising a polynucleotide construct comprising a polynucleotide molecule operably linked to a promoter that drives expression in the plant, wherein said polynucleotide molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- and
- (c) a nucleotide sequence that is the complement of the nucleotide sequence of (a) (b).
An isolated polynucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- and
- (c) a nucleotide sequence that is the complement of the nucleotide sequence of (a) or (b).
An expression cassette comprising a polynucleotide molecule, said polynucleotide molecule comprising a nucleotide seguence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 3 or 4;
- (b) a nucleotide sequence encoding an amino acid sequence comprising SEQ ID NO:
- 2;
- and
- (c) a nucleotide sequence that is the complement of the nucleotide sequence of (a) or (b).
Dependencies are inferred from the claim text.