Bacillus thuringiensis cry gene and protein
AssigneesPioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and Company
InventorsAndre R. Abad, Ronald D. Flannagan, Billy F. McCutchen, Cao Guo Yu
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Abstract
Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.
Claims20
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:1;
- b) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:2;
- and
- c) a nucleotide sequence encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:2, wherein said polypeptide has pesticidal activity.
A transformed plant cell comprising at least one polynucleotide construct that comprises a heterologous nucleotide sequence operably linked to a promoter that drives expression in the plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:
- 1;
- b) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 2;
- and
- c) a nucleotide sequence encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:2, wherein said polypeptide has pesticidal activity.
A plant comprising at least one polynucleotide construct that comprises a heterologous nucleotide sequence operably linked to a promoter that drives expression in the plant, wherein said nucleotide sequence is selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:
- 1;
- b) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 2;
- and
- c) a nucleotide sequence encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:2, wherein said polypeptide has pesticidal activity.
A method for protecting a plant from an insect pest, said method comprising introducing into said plant at least one polynucleotide construct that comprises a heterologous nucleotide sequence operably linked to a promoter that drives expression in the plant, wherein said nucleotide sequence is selected from the group consisting of:
- a) the nucleotide sequence set forth in SEQ ID NO:1;
- b) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 2;
- and
- c) a nucleotide sequence encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:2, wherein said polypeptide has pesticidal activity.
Dependencies are inferred from the claim text.