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US 7,803,988 B2Granted
Plant transformation method
AssigneeBiogemma
InventorsThierry Risacher, Melanie Craze
Life of this patent
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Abstract
The invention provides a transformation method comprising inoculation and co-cultivation of a target tissue, from a target plant, with Agrobacterium, at a time when the target tissue is in its natural plant environment, followed by generation of a transgenic plant via dedifferentiation and regeneration of the target tissue.
Claims17
A method of generating a transgenic monocotyledonous plant comprising;
- inoculating and co-cultivating a target tissue, from a target monocotyledonous plant, with Agrobacterium, at a time when said target tissue is in its natural plant environment;
- removing said target tissue from its natural environment;
- and generating a transgenic plant via dedifferentiation and regeneration of said target tissue, wherein at least some of the dedifferentiation of the target tissue is carried out in vitro;
- wherein said target tissue is an embryo and the target area for inoculation is the interface between developing scutellum surface and adjoining starch parenchyma of endosperm.
1 more element
A method of transforming monocotyledonous plant tissue comprising;
- inoculating and co-cultivating a target tissue, from a target monocotyledonous plant, with Agrobacterium, at a time when said target tissue is in its natural plant environment;
- removing said target tissue from its natural environment;
- and generating dedifferentiated tissue from the target tissue;
- wherein at least some of the dedifferentiation of the target tissue is carried out in vitro, wherein said target tissue is an embryo and the target area for inoculation is the interface between the developing scutellum surface and adjoining starch parenchyma of endosperm.
1 more element
Dependencies are inferred from the claim text.