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US 7,803,986 B2Granted
Fructan Biosynthetic Enzymes
AssigneeE. I. Du Pont de Nemours and Company
InventorJohan M. Stoop
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Abstract
This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.
Claims11
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having 1-SST activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:16 have at least 98% sequence identity based on the Clustal alignment method with default parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, or
- (b) a complement of the nucleotide sequence of (a), wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary.
Dependencies are inferred from the claim text.