Compositions and methods for controlling plant parasitic nematodes
AssigneeKansas State University Research Foundation
InventorsHarold N. Trick, Judith L. Roe, Timothy C. Todd, Michael A. Herman
Life of this patent
Estimating the term…
Abstract
The present invention relates to compositions and methods for controlling nematode infestation of plants. In particular, the present invention provides vectors comprising sequences designed to control nematodes by RNA interference (RNAi) and transgenic plants transformed with such vectors.
Claims17
A transgenic plant comprising a nucleic acid sequence having a sense sequence linked to its complementary antisense sequence and encoding a double stranded RNA that inhibits expression of a Heterodera glycines embryonic lethal phenotype gene, wherein the sense or antisense sequence comprises SEQ ID NO:
- 1, SEQ ID NO:
- 9 or SEQ ID NO:
- 18, wherein nematodes ingesting said double stranded nematode RNA do not proliferate, and wherein said transgenic plant exhibits increased resistance to soybean cyst nematodes as compared to a control plant.
A vector comprising a nucleic acid sequence having a sense sequence linked to its complementary anstisense and encoding a double stranded RNA sequence that inhibits expression of a Heterodera glycines embryonic lethal phenotype gene, wherein said sense sequence or antisense sequence comprises SEQ ID NO:
- 1, SEQ ID NO:
- 9 or SEQ ID NO:
- 18;
- and wherein nematodes ingesting said double stranded nematode RNA do not proliferate.
A method for controlling Heterodera glycines comprising providing a transgenic soybean plant comprising a nucleic acid sequence having a sense sequence linked to its complementary anstisense and encoding a double stranded RNA that inhibits expression of a Heterodera glycines embryonic lethal phenotype gene, wherein the proliferation of nematodes feeding on said plant is reduced as compared to nematodes feeding on non-transgenic plant and wherein said sense sequence or antisense sequence comprises SEQ ID NO:
- 1, SEQ ID NO:9 and SEQ ID NO:18.
A transgenic plant comprising a nucleic acid encoding a double stranded RNA that causes decreased proliferation of soybean cyst nematodes feeding on said transgenic plant as compared to soybean cyst nematodes feeding on non-transgenic plants, wherein one strand of said double stranded RNA is complementary to a nematode embryonic lethal phenotype gene, and wherein said one strand comprises a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 9 and SEQ ID NO:
- 18.
Dependencies are inferred from the claim text.