Nucleic acid adsorption under low-salt conditions
AssigneeRoche Diagnostics Operations, Inc.
InventorChristian Birkner
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Abstract
Biscationic organic compounds are disclosed which promote adsorption of nucleic acids from an aqueous solution to a solid phase such as silica. Adsorption takes place under low salt conditions. Further disclosed are methods and kits suitable for nucleic acid isolation from aqueous solutions.
Claims7
A method for binding a nucleic acid to a solid phase comprising the steps of
- combining the solid phase and an aqueous solution comprising the nucleic acid, a biscationic organic compound, and a buffer salt, wherein the solution has a salt concentration exclusive of the biscationic organic compound between 5 and 300 mM under conditions suitable for binding of the nucleic acid to the solid phase.
An aqueous solution useful for adsorbing a nucleic acid to a solid phase, the solution comprising the nucleic acid, a biscationic organic compound, and a buffer salt, wherein the solution has a salt concentration exclusive of the biscationic organic compound between 5 and 300 mM, wherein the biscationic compound is selected from the group consisting of a bis-benzimidazolium cation, a bis-imidazolium cation, and guanidine, N,N′″-1,4-butanediyl cation.
A method for purifying a nucleic acid comprising the steps of
- contacting a solid phase capable of reversibly binding the nucleic acid with an aqueous solution comprising the nucleic acid, a biscationic organic compound, and a buffer salt, wherein the solution has a salt concentration exclusive of the biscationic organic compound between 5 and 300 mM under conditions whereby the nucleic acid binds to the solid phase,
- separating the solid phase with the bound nucleic acid from the solution, and
- eluting the nucleic acid from the solid phase, thereby purifying the nucleic acid.
A kit of parts for isolating a nucleic acid, the kit comprising a solid phase capable of reversibly binding the nucleic acid and a reagent comprising a buffered solution of a biscationic compound selected from the group consisting of a bis-benzimidazolium cation, a bis-imidazolium cation, and guanidine, N,N′″-1,4-butanediyl cation.
Dependencies are inferred from the claim text.