Methods and compositions for expression of transgenes in plants
AssigneeMonsanto Technology LLC
InventorsAlan L. Kriz, Michael H. Luethy, Dale A. Voyles
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Abstract
Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus Coix for transformation. Included in these transgene sequences are Coix promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.
Claims23
An isolated DNA molecule that exhibits terminator activity comprising a nucleic acid sequence selected from the group consisting of:
- (a) a polynucleotide sequence comprising SEQ ID NO:17;
- (b) a polynucleotide sequence comprising a fragment of SEQ ID NO:
- 17, wherein the fragment comprises at least 50 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:
- 17:
- and (c) a polynucleotide sequence fully complementary to (a) or (b).
Dependencies are inferred from the claim text.