Methods and compositions for expression of transgenes in plants
AssigneeMonsanto Technology LLC
InventorsAlan L. Kriz, Michael H. Luethy, Dale A. Voyles
Life of this patent
Estimating the term…
Abstract
Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus Coix for transformation. Included in these transgene sequences are Coix promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.
Claims12
A DNA molecule that exhibits terminator activity comprising a nucleic acid sequence selected from the group consisting of:
- (a) a polynucleotide sequence comprising SEQ ID NO:
- 11;
- (b) a polynucleotide sequence comprising a fragment of SEQ ID NO:
- 11, wherein said fragment exhibits terminator activity;
- and (c) a polynucleotide sequence fully complementary to (a) or (b);
- wherein the DNA molecule is operably linked to a heterologous transcribable polynucleotide.
Dependencies are inferred from the claim text.