Polynucleotides encoding myostatin binding agents
AssigneeAmgen Inc.
InventorsHq Han, Hosung Min, Thomas C. Boone
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Abstract
The present invention provides binding agents comprising peptides capable of binding myostatin and inhibiting its activity. In one embodiment the binding agent comprises at least one myostatin-binding peptide attached directly or indirectly to at least one vehicle such as a polymer or an Fc domain. The binding agents of the present invention produced increased lean muscle mass when administered to animals and decreased fat to muscle ratios. Therapeutic compositions containing the binding agents of the present invention are useful for treating muscle-wasting disorders and metabolic disorders including diabetes and obesity.
Claims15
An isolated nucleic acid molecule comprising a polynucleotide encoding a myostatin binding agent comprising at least one peptide capable of binding myostatin, wherein the peptide comprises the sequence Cb1b2Wb3WMCPP (SEQ ID NO:
- 353), wherein
- b1 is selected from any one of the amino acids T, I, or R;
- b2 is selected from any one of R, S, Q, and
- b3 is selected from any one of P, R and Q.
An isolated nucleic acid molecule comprising a polynucleotide encoding a myostatin binding agent wherein said agent has the structure:
- (X1)a-F1-(X2)b, or multimers thereof;
- wherein F1 is an Fc domain, and X1 and X2 are each independently selected from
- -(L1)c-P1;
- -(L1)c-P1-(L2)d-P2;
- -(L1)c-P1-(L2)d-P2-(L3)e-P3;
- and -(L1)c-P1-(L2)d-P2-(L3)e-P3-(L4)f-P4;
- wherein P1, P2, P3, and P4 are peptides capable of binding myostatin, and each comprise the sequence Cb1b2Wb3WMCPP (SEQ ID NO:
- 353), wherein
- b1 is selected from any one of the amino acids T, I, or R;
- b2 is selected from any one of R, S, Q;
- b3 is selected from any one of P, R and Q,
- wherein L1, L2, L3, and L4 are each peptide linkers;
- and wherein a, b, c, d, e, and f are each independently 0 or 1, provided that at least one of a and b is 1.
Dependencies are inferred from the claim text.