US 7,803,907 B2Granted

c-MET kinase binding proteins

AssigneeAmgen Mountain View Inc.

InventorsWillem P. C. Stemmer, D. Victor Perlroth, Sanjeev Satyal, Benjamin M. Alba, Alice Bakker, Amy N. Duguay, Qiang Liu, Joshua Silverman, Richard Smith, Candace Swimmer

Life of this patent

Estimating the term…

Abstract

Polypeptides comprising monomer domains that bind to c-MET, or portions thereof, are provided.

Claims19

Dependent claims

An isolated polypeptide comprising at least one monomer domain that binds to c-MET, wherein the at least one monomer domain comprises a sequence selected from the group consisting of (i) SEQ ID NO:8, (ii) SEQ ID NO:13, (iii) at least amino acids 1-34 of SEQ ID NO:15 and (iv) a sequence that is at least 95% identical to a sequence defined by any of (i), (ii) or (iii), as defined herein:

Cxxx[EQ]FxCxSTxRC[IV]xxxWxCDGDNDCEDxSDEx (SEQ ID NO:8),
wherein the first x is an amino acid chosen from A, L, E, R, P, and Q,
the second x is an amino acid chosen from P, A, and S,
the third x is an amino acid chosen from S, N, and G,
the fourth x is an amino acid chosen from T, Q, and K,
the fifth x is an amino acid chosen from N, R, S, and H,
the sixth x is an amino acid chosen from G, N, D, and E,
the seventh x is an amino acid chosen from P and S,
the eighth x is an amino acid chosen from Q, L, and A,
the ninth x is an amino acid chosen from E, T, Q, A, S, G, and D
the tenth x is an amino acid chosen from V and L,
the eleventh x is an amino acid chosen from S and G,
the twelfth x is an amino acid chosen from A, S, and K, and
amino acids in brackets are alternative amino acids at a single position;
Cxxx[EQ]FxCxSTGRCxPxxWxCxGxNDCEDxSDEx (SEQ ID NO:13),
wherein the first x is an amino acid chosen from A, E, V, R, L, P, and Q,
the second x is an amino acid chosen from A, S, P, T, and L,
the third x is an amino acid chosen from D, S, N, and G,
the fourth x is an amino acid chosen from Q, T, and R,
the fifth x is an amino acid chosen from N, H, and R,
the sixth x is any amino acid,
the seventh x is an amino acid chosen from V, Q, L, R, A, and G,
the eighth x is an amino acid chosen from S, E, D, Q, and A,
the ninth x is any amino acid,
the tenth x is amino acid chosen from D and H,
the eleventh amino acid is V and D,
the twelfth x is an amino acid chosen from S and G,
the thirteenth x is an amino acid chosen from A, T, S, and E,
and amino acids in brackets are alternative amino acids at a single position;
and
at least amino acids 1-34 of
Cxxx[EQ]FxCxxxxxC[ILV]xxxWxCDGxNDCxDxSxExxxxC (SEQ ID NO:15),
wherein the first x is an amino acid chosen from P, Q, V, L, and E,
the second x is an amino acid chosen from A, S, and P,
the third x is an amino acid chosen from G, S, N, and D,
the fourth x is an amino acid chosen from Q, R, T, and M,
the fifth x is any amino acid,
the sixth x is any amino acid,
the seventh x is an amino acid chosen from T, G, and N,
the eighth x is any amino acid,
the ninth x is an amino acid chosen from R and Q,
the tenth x is amino acid chosen from P and S,
the eleventh amino acid is any amino acid,
the twelfth x is an amino acid chosen from T, P, N, D, A, and H,
the thirteenth x is an amino acid chosen from L, G, and V,
the fourteenth x is an amino acid chosen from D, V, and E,
the fifteenth x is an amino acid chosen from E and G,
the sixteenth x is an amino acid chosen from G and S,
the seventeenth x is any amino acid,
the eighteenth x is any amino acid,
the nineteenth x is any amino acid,
the twentieth x is any amino acid,
the twenty first x is any amino acid,
and amino acids in brackets are alternative amino acids at a single position.

51 more elements

An isolated polypeptide comprising at least three monomer domains that bind to c-MET, wherein

one monomer domain comprises a sequence selected from the group consisting of
(1) Cxxx[EQ]FxCxSTGRCxPxxWxCxGxNDCEDxSDEx (SEQ ID NO:13),
wherein the first x is an amino acid chosen from A, E, V, R, L, F, and Q,
the second x is an amino acid chosen from A, S, P, T, and L,
the third x is an amino acid chosen from D, S, N, and G,
the fourth x is an amino acid chosen from Q, T, and R,
the fifth x is an amino acid chosen from N, H, and R,
the sixth x is any amino acid,
the seventh x is an amino acid chosen from V, Q, L, R, A, and G,
the eighth x is an amino acid chosen from S, E, D, Q, and A,
the ninth x is any amino acid,
the tenth x is amino acid chosen from D and H,
the eleventh amino acid is V and D,
the twelfth x is an amino acid chosen from S and G,
the thirteenth x is an amino acid chosen from A, T, S, and E,
and amino acids in brackets are alternative amino acids at a single position, and
(ii) a sequence that is at least 95% identical to a sequence defined by (i);
another monomer domain comprises a sequence selected from the group consisting of
(iii) at least amino acids 1-34 of:
Cxxx[EQ]FxCxxxxxC[ILV]xxxWxCDGxNDCxDxSxExxxxC (SEQ ID NO:15),
wherein the first x is an amino acid chosen from P, Q, V, L, and E,
the second x is an amino acid chosen from A, S, and P,
the third x is an amino acid chosen from G, S, N, and D,
the fourth x is an amino acid chosen from Q, R, T, and M,
the fifth x is an amino acid chosen from any amino acid,
the sixth x is any amino acid,
the seventh x is an amino acid chosen from T, G, and N,
the eight x is any amino acid,
the ninth x is an amino acid chosen from R and Q,
the tenth x is amino acid chosen from P and S,
the eleventh amino acid is any amino acid,
the twelfth x is an amino acid chosen from T, P, N, D, A, and H,
the thirteenth x is an amino acid chosen from L, G, and V,
the fourteenth x is an amino acid chosen from D, V, and E,
the fifteenth x is an amino acid chosen from E and G,
the sixteenth x is an amino acid chosen from G and S,
the seventeenth x is any amino acid,
The eighteenth x is any amino acid,
the nineteenth x is any amino acid,
the twentieth x is any amino acid,
the twenty first x is any amino acid,
and amino acids in brackets are alternative amino acids at a single position, and
(iv) a sequence that is at least 95% identical to a sequence defined by (iii);
and
yet another monomer domain comprises a sequence selected from the group consisting of
(v) at least amino acids 1-34 of:
Cxxx[EQ]FxCxxxxxC[ILV]xxxWxCDGxNDCxDxSxExxxxC (SEQ ID NO:15),
wherein the first x is an amino acid chosen from P, Q, V, L, and E,
the second x is an amino acid chosen from A, S, and P,
the third x is an amino acid chosen from G, S, N, and D,
the fourth x is an amino acid chosen from Q, R, T, and M,
the fifth x is an amino acid chosen from any amino acid,
the sixth x is any amino acid,
the seventh x is an amino acid chosen from T, G, and N,
the eighth x is any amino acid,
the ninth x is an amino acid chosen from R and Q,
the tenth x is amino acid chosen from P and S,
the eleventh amino acid is any amino acid,
the twelfth x is an amino acid chosen from T, P, N, D, A, and H,
the thirteenth x is an amino acid chosen from L, G, and V,
the fourteenth x is an amino acid chosen from D, V, and E,
the fifteenth x is an amino acid chosen from E and G,
the sixteenth x is an amino acid chosen from G and S,
the seventeenth x is any amino acid,
the eighteenth x is any amino acid,
the nineteenth x is any amino acid,
the twentieth x is any amino acid,
the twenty first x is any amino acid,
and amino acids in brackets are alternative amino acids at a single position;
and
(vi) a sequence that is at least 95% identical to a sequence defined by (v).

68 more elements

An isolated polypeptide comprising at least three monomer domains that bind to c-MET, wherein

one monomer domain comprises at least amino acids 1-38 of:
CQPNEFQCHSTGRCIPASWLCDGDNDCEDSSDESPANCATPTHT (SEQ ID NO:641);
another monomer domain comprises a sequence selected from the group consisting of
(i) at least amino acids 1-34 of:
Cxxx[EQ]FxCxxxxxC[ILV]xxxWxCDGxNDCxDxSxExxxC (SEQ ID NO:15),
wherein the first x is an amino acid chosen from P, Q, V, L, and E,
the second x is an amino acid chosen from A, S, and P,
the third x is an amino acid chosen from G, S, N, and D,
the fourth x is an amino acid chosen from Q, R, T, and M,
the fifth x is an amino acid chosen from any amino acid,
the sixth x is any amino acid,
the seventh x is an amino acid chosen from T, G, and N,
the eighth x is any amino acid,
the ninth x is an amino acid chosen from R and Q,
the tenth x is amino acid chosen from P and S,
the eleventh amino acid is any amino acid,
the twelfth x is an amino acid chosen from T, P, N, D, A, and H,
the thirteenth x is an amino acid chosen from L, G, and V,
the fourteenth x is an amino acid chosen from D, V, and E,
the fifteenth x is an amino acid chosen from E and G,
the sixteenth x is an amino acid chosen from G and S,
the seventeenth x is any amino acid,
the eighteenth x is any amino acid,
the nineteenth x is any amino acid,
the twentieth x is any amino acid,
the twenty first x is any amino acid,
and amino acids in brackets are alternative amino acids at a single position, and
(ii) a sequence that is at least 95% identical to a sequence defined by (i);
and
yet another monomer domain comprises a sequence selected from the group consisting of
(iii) at least amino acids 1-34 of:
Cxxx[EQ]FxCxxxxxC[ILV]xxxWxCDGxNDCxDxSxExxxxC (SEQ ID NO:15),
wherein the first x is an amino acid chosen from P, Q, V, L, and E,
the second x is an amino acid chosen from A, S, and P,
the third x is an amino acid chosen from G, S, N, and D,
the fourth x is an amino acid chosen from Q, R, T, and M,
the fifth x is an amino acid chosen from any amino acid,
the sixth x is any amino acid,
the seventh x is an amino acid chosen from T, G, and N,
the eighth x is any amino acid,
the ninth x is an amino acid chosen from R and Q,
the tenth x is amino acid chosen from P and S,
the eleventh amino acid is any amino acid,
the twelfth x is an amino acid chosen from T, P, N, D, A, and H,
the thirteenth x is an amino acid chosen from L, G, and V,
the fourteenth x is an amino acid chosen from D, V, and E,
the fifteenth x is an amino acid chosen from E and G,
the sixteenth x is an amino acid chosen from G and S,
the seventeenth x is any amino acid,
the eighteenth x is any amino acid,
the nineteenth x is any amino acid,
the twentieth x is any amino acid,
the twenty first x is any amino acid,
and amino acids in brackets are alternative amino acids at a single position;
and
(iv) a sequence that is at least 95% identical to a sequence defined by (iii).

53 more elements

Dependencies are inferred from the claim text.

Facts

Application: 11/155,989Filed 17 Jun 2005 · continuation in part
Priority: 17 Jun 2004
Published: US 2006/0008844 A110 Aug 2006
Granted: 28 Sep 2010
Term ends: Not estimatedThe source records don’t support an estimate.

Classification14 codes

C07K 14/47
From vertebrates: from mammals
A61P 35/00
Antineoplastic agents
A61P 35/02
Antineoplastic agents: specific for leukemia
C07K 14/705
From animals; from humans: receptors; Cell surface antigens; Cell surface determinants

Family

Application10/871,602Parent of this continuation-in-part · filed 17 Jun 2004
Application10/957,351Parent of this continuation-in-part · filed 30 Sep 2004
US 2006/0177831 A1Pre-grant publication · published 10 Aug 2006
US 2005/0089932 A1Published application
US 2006/0008844 A1Published application
US 7,803,907 B2This patent

From the US filing history. Foreign family members aren’t listed.

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Cxxx[EQ]FxCxSTxRC[IV]xxxWxCDGDNDCEDxSDEx (SEQ ID NO:8),
wherein the first x is an amino acid chosen from A, L, E, R, P, and Q,
the second x is an amino acid chosen from P, A, and S,
the third x is an amino acid chosen from S, N, and G,
the fourth x is an amino acid chosen from T, Q, and K,
the fifth x is an amino acid chosen from N, R, S, and H,
the sixth x is an amino acid chosen from G, N, D, and E,
the seventh x is an amino acid chosen from P and S,
the eighth x is an amino acid chosen from Q, L, and A,
the ninth x is an amino acid chosen from E, T, Q, A, S, G, and D
the tenth x is an amino acid chosen from V and L,
the eleventh x is an amino acid chosen from S and G,
the twelfth x is an amino acid chosen from A, S, and K, and
amino acids in brackets are alternative amino acids at a single position;
Cxxx[EQ]FxCxSTGRCxPxxWxCxGxNDCEDxSDEx (SEQ ID NO:13),
wherein the first x is an amino acid chosen from A, E, V, R, L, P, and Q,
the second x is an amino acid chosen from A, S, P, T, and L,
the third x is an amino acid chosen from D, S, N, and G,
the fourth x is an amino acid chosen from Q, T, and R,
the fifth x is an amino acid chosen from N, H, and R,
the sixth x is any amino acid,
the seventh x is an amino acid chosen from V, Q, L, R, A, and G,
the eighth x is an amino acid chosen from S, E, D, Q, and A,
the ninth x is any amino acid,
the tenth x is amino acid chosen from D and H,
the eleventh amino acid is V and D,
the twelfth x is an amino acid chosen from S and G,
the thirteenth x is an amino acid chosen from A, T, S, and E,
and amino acids in brackets are alternative amino acids at a single position;
and
at least amino acids 1-34 of
Cxxx[EQ]FxCxxxxxC[ILV]xxxWxCDGxNDCxDxSxExxxxC (SEQ ID NO:15),
wherein the first x is an amino acid chosen from P, Q, V, L, and E,
the second x is an amino acid chosen from A, S, and P,
the third x is an amino acid chosen from G, S, N, and D,
the fourth x is an amino acid chosen from Q, R, T, and M,
the fifth x is any amino acid,
the sixth x is any amino acid,
the seventh x is an amino acid chosen from T, G, and N,
the eighth x is any amino acid,
the ninth x is an amino acid chosen from R and Q,
the tenth x is amino acid chosen from P and S,
the eleventh amino acid is any amino acid,
the twelfth x is an amino acid chosen from T, P, N, D, A, and H,
the thirteenth x is an amino acid chosen from L, G, and V,
the fourteenth x is an amino acid chosen from D, V, and E,
the fifteenth x is an amino acid chosen from E and G,
the sixteenth x is an amino acid chosen from G and S,
the seventeenth x is any amino acid,
the eighteenth x is any amino acid,
the nineteenth x is any amino acid,
the twentieth x is any amino acid,
the twenty first x is any amino acid,
and amino acids in brackets are alternative amino acids at a single position.

51 more elements

Facts

Application

11/155,989Filed 17 Jun 2005 · continuation in part

Priority

17 Jun 2004

Published

US 2006/0008844 A110 Aug 2006

Granted

28 Sep 2010

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

C07K 14/47
From vertebrates: from mammals
A61P 35/00
Antineoplastic agents
A61P 35/02
Antineoplastic agents: specific for leukemia
C07K 14/705
From animals; from humans: receptors; Cell surface antigens; Cell surface determinants

Family

Application10/871,602Parent of this continuation-in-part · filed 17 Jun 2004

Application10/957,351Parent of this continuation-in-part · filed 30 Sep 2004

US 2006/0177831 A1Pre-grant publication · published 10 Aug 2006

US 2005/0089932 A1Published application

US 2006/0008844 A1Published application

US 7,803,907 B2This patent

From the US filing history. Foreign family members aren’t listed.