Compositions and methods for detecting phosphomonoester
AssigneeIllumina, Inc.
InventorsIgor Kozlov, Peter Melnyk, Chanfeng Zhao
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Abstract
The invention provides a method of modifying a phosphomonoester moiety of a target compound. The method can include the steps of (a) providing a target compound having an electrophilic moiety and a phosphomonoester moiety; (b) contacting the target compound with a first carbodiimide compound under conditions for preferential addition of the first carbodiimide compound to the electrophilic moiety over the phosphomonoester moiety, thereby forming an electrophile-protected target compound; and (c) contacting the electrophile-protected target compound with a second carbodiimide compound and a nucleophilic compound under conditions for addition of the nucleophilic compound to the phosphomonoester.
Claims38
A method of detecting a phosphomonoester moiety of a target compound, comprising
- (a) providing a target compound comprising a carboxylic acid moiety and a phosphomonoester moiety, wherein said target compound comprises a protein;
- (b) contacting said target compound with a first carbodiimide compound under conditions for preferential addition of said first carbodiimide compound to said carboxylic acid moiety over said phosphomonoester moiety, thereby forming a carboxyl-protected target compound;
- (c) contacting said carboxyl-protected target compound with a second carbodiimide compound and an amine under conditions for addition of said amine to said phosphomonoester moiety, thereby forming a nucleophile-modified phosphate moiety;
- and
- (d) detecting said nucleophile-modified phosphate moiety.
Dependencies are inferred from the claim text.