Method for detecting target compounds containing sulfenic acids using new reagents
AssigneeWake Forest University Health Sciences
InventorsLeslie B. Poole, S. Bruce King, Jacquelyn S. Fetrow
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Abstract
The present invention provides compounds of Formula I: wherein: R1 is a label (e.g., a detectable groups; an anti-tumor agent)s; L is present or absent and when present is a linking group; and x represents an integer from 1 to 10; or a pharmaceutically acceptable salt thereof. the compounds are useful for, among other things, identifying cysteine sulfenic acids in proteins and monitoring oxidative damage in proteins and cells.
Claims22
A method of determining whether a target compound containing a sulfenic acid is present or absent in a sample suspected of containing the same, comprising:
- contacting said sample with a compound of Formula I:
- wherein:
- R1 is a label, wherein said label is selected from the group consisting of biotin, fluorophores, antigens, porphyrins, and stable or radioactive isotopes;
- L is present or absent and when present is a linking group;
- and
- x represents an integer from 1 to 10;
- to form a bound target compound containing sulfenic acid, if present;
- separating said bound target compound, if present, from unbound compound of Formula I, to form a fraction that may comprise said bound target compound;
- and then
- detecting the presence or absence of said label in said fraction,
- the presence of said label in said fraction indicating the presence of said target compound containing a sulfenic acid in said sample.
A method of determining whether a protein containing a sulfenic acid is present or absent in a sample suspected of containing the same, comprising:
- contacting said sample with a compound of Formula I:
- wherein:
- R1 is a label, wherein said label is selected from the group consisting of biotin, fluorophores, antigens, porphyrins, and stable or radioactive isotopes;
- L is present or absent and when present is a linking group;
- and
- x represents an integer from 1 to 10;
- to form a bound protein containing sulfenic acid, if present;
- separating said bound protein, if present, from unbound compound of Formula I, to form a fraction that may comprise said bound protein;
- and then
- detecting the presence or absence of said label in said fraction,
- the presence of said label in said fraction indicating the presence of said protein containing a sulfenic acid in said sample.
Dependencies are inferred from the claim text.