Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate
AssigneesE.I. du Pont de Nemours and Company, Alliance for Sustainable Energy, LLC
InventorsPerry G. Caimi, Yat-Chen Chou, Mary Ann Franden, Kyle Knoke, Luan Tao, Paul V. Viitanen, Min Zhang, Yuying Zhang
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Abstract
Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.
Claims13
A recombinant microorganism of the genus Zymomonas which utilizes xylose to produce ethanol by fermentation in a mixed sugar medium, said microorganism comprising at least one genetic modification which reduces expression of the endogenous integration host factor alpha subunit (HimA) protein encoded by a himA gene.
Dependencies are inferred from the claim text.