Adeno-associated virus vectors
AssigneeUniversity of Iowa Research Foundation
InventorsJohn F. Engelhardt, Dongsheng Duan
Life of this patent
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Abstract
The invention provides an isolated and purified DNA molecule comprising at least one DNA segment, a biologically active subunit or variant thereof, of a circular intermediate of adeno-associated virus, which DNA segment confers increased episomal stability, persistence or abundance of the isolated DNA molecule in a host cell. The invention also provides a composition comprising at least two adeno-associated virus vectors.
Claims12
A composition comprising:
- a) a first adeno-associated virus vector comprising linked:
- i) a first DNA segment comprising a 5′-inverted terminal repeat of adeno-associated virus;
- ii) a second DNA segment comprising a promoter operably linked to a DNA fragment comprising an exon of a gene and a splice donor site, wherein the second DNA segment does not encode a full-length polypeptide;
- and
- iii) a third DNA segment comprising a 3′-inverted terminal repeat of adeno-associated virus;
- and
- b) a second adeno-associated virus vector comprising linked:
- i) a first DNA segment comprising a 5′-inverted terminal repeat of adeno-associated virus;
- ii) a second DNA segment comprising a splice acceptor site and a DNA fragment with at least one other exon which together with the DNA segment of (a)(ii) encodes a full-length polypeptide;
- and
- iii) a third DNA segment comprising a 3′-inverted terminal repeat of adeno-associated virus.
A method to transfer at least a portion of an open reading frame to a host cell comprising contacting the host cell with a first adeno-associated virus vector comprising linked:
- i) a first DNA segment comprising a 5′-inverted terminal repeat of adeno-associated virus;
- ii) a second DNA segment comprising a promoter operably linked to a DNA fragment comprising an exon of a gene and a splice donor site, wherein the second DNA segment does not encode a full-length polypeptide;
- and
- iii) a third DNA segment comprising a 3′-inverted terminal repeat of adeno-associated virus.
Dependencies are inferred from the claim text.