Recombinant tumor specific antibody and use thereof
AssigneeMerck Patent GmbH
InventorsStephen D. Gillies, Kin-Ming Lo, Susan Xiuqi Qian
Life of this patent
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Abstract
The invention provides a family of antibodies that specifically bind the human epithelial cell adhesion molecule. The antibodies comprise modified variable regions, more specially, modified framework regions, which reduce their immunogenicity when administered to a human. The antibodies, when coupled to the appropriate moiety, may be used in the diagnosis, prognosis and treatment of cancer.
Claims19
A nucleic acid encoding an anti-EpCAM protein comprising an antibody that binds human EpCAM and that comprises:
- (a) an immunoglobulin light chain variable region comprising:
- (i) amino acid residues 24-31 of SEQ ID NO:
- 1;
- (ii) amino acid residues 49-55 of SEQ ID NO:
- 1;
- and (iii) amino acid residues 88-96 of SEQ ID NO:
- 1;
- and
- (b) an amino acid sequence defining an immunoglobulin light chain framework region selected from the group consisting of:
- (i) amino acid residues 1-23 of SEQ ID NO:8;
- and (ii) amino acid residues 1-23 of SEQ ID NO:9.
A nucleic acid encoding an anti-EpCAM protein, wherein the protein comprises an antibody that binds human EpCAM and that comprises:
- (a) an immunoglobulin heavy chain variable region comprising:
- (i) amino acid residues 26-35 of SEQ ID NO:2;
- (ii) amino acid residues 50-62 of SEQ ID NO:2;
- and (iii) amino acid residues 101-105 of SEQ ID NO:2;
- and
- (b) an amino acid sequence defining an immunoglobulin heavy chain framework region selected from the group consisting of:
- (i) amino acid residues 1-25 of SEQ ID NO:
- 18;
- and (ii) amino acid residues 67-98 of SEQ ID NO:
- 18.
A nucleic acid encoding an anti-EpCAM protein, wherein the protein comprises an antibody that binds human EpCAM and that comprises an antibody light chain variable region comprising an amino acid sequence defined by residues 1-106 of SEQ ID NO:9 and an antibody heavy chain variable region comprising an amino acid sequence defined by residues 1-116 of SEQ ID NO:
- 18.
A nucleic acid encoding a humanized or deimmunized anti-EpCAM protein suitable for administering into a human patient for treating a disease associated with EpCAM over-expression, wherein the protein comprises an antibody that binds human EpCAM and that comprises:
- (a) an immunoglobulin light chain variable region comprising:
- (i) amino acid residues 24-31 of SEQ ID NO:
- 1;
- (ii) amino acid residues 49-55 of SEQ ID NO:
- 1;
- and (iii) amino acid residues 88-96 of SEQ ID NO:
- 1;
- and
- (b) an amino acid sequence defining an immunoglobulin light chain framework region selected from the group consisting of:
- (i) amino acid residues 1-23 of SEQ ID NO:8;
- and (ii) amino acid residues 1-23 of SEQ ID NO:9.
A nucleic acid encoding a humanized or deimmunized anti-EpCAM protein suitable for administering into a human patient for treating a disease associated with EpCAM over-expression, wherein the protein comprises an antibody that binds human EpCAM and that comprises:
- (a) an immunoglobulin heavy chain variable region comprising:
- (i) amino acid residues 26-35 of SEQ ID NO:2;
- (ii) amino acid residues 50-62 of SEQ ID NO:2;
- and (iii) amino acid residues 101-105 of SEQ ID NO:2;
- and
- (b) an amino acid sequence defining an immunoglobulin heavy chain framework region selected from the group consisting of:
- (i) amino acid residues 1-25 of SEQ ID NO:
- 18;
- and (ii) amino acid residues 67-98 of SEQ ID NO:
- 18.
A nucleic acid encoding a humanized or deimmunized anti-EpCAM protein suitable for administering into a human patient for treating a disease associated with EpCAM over-expression, wherein the protein comprises an antibody that binds human EpCAM and that comprises an antibody light chain variable region comprising an amino acid sequence defined by residues 1-106 of SEQ ID NO:9 and an antibody heavy chain variable region comprising an amino acid sequence defined by residues 1-116 of SEQ ID NO:18.
Dependencies are inferred from the claim text.