Medium supplement and animal cell culture medium
AssigneeSeiren Co., Ltd.
InventorsMasahiro Sasaki, Hideyuki Yamada, Katsue Osada, Satoshi Terada
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Abstract
An object of the present invention is to provide a medium supplement for animal cell culture and an animal cell culture medium. The present invention relates to a medium supplement for animal cell culture comprising sericin or a sericin derivative and an animal cell culture medium comprising at least said medium supplement and a basal medium composition.
Claims2
A method of culturing animal cells, comprising the steps of culturing animal cells using a medium for animal cell culture to grow said animal cells, wherein the medium for animal cell culture comprises:
- a basal medium composition for animal cell culture;
- and
- sericin comprising at least a polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 2 or a sericin derivative comprising at least a polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 1 as an essential region, or at least a polypeptide consisting of a modified amino acid sequence of SEQ ID NO:
- 2 in which one or more amino acid residues are conservatively substituted wherein the sericin or the sericin derivative is mixed with the basal medium and wherein the growth of the animal cells is stimulated by the sericin or the sericin derivative.
A method of producing a protein of interest, comprising the steps of culturing animal cells capable of producing the protein of interest using a medium for animal cell culture, and recovering the produced protein from said medium and/or said animal cells, wherein the medium for animal cell culture comprises:
- a basal medium composition for animal cell culture;
- and
- sericin comprising at least a polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 2 or a sericin derivative comprising at least a polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 1 as an essential region, or at least a polypeptide consisting of a modified amino acid sequence of SEQ ID NO:
- 2 in which one or more amino acid residues are conservatively substituted wherein the sericin or the sericin derivative is mixed with the basal medium and wherein the growth of the animal cells is stimulated by the sericin or the sericin derivative.
Dependencies are inferred from the claim text.