Method, apparatus and system for separating eucaryotic or procaryotic cells or other particularly biological material from a suspension

A method for separating eukaryotic cells, procaryotic cells, or other biological material which is sensitive to high shearing forces from a suspension, the method comprising the following steps:

• providing at least one hydrocyclone with an inlet and an outlet, the outlet being essentially opposite to the inlet wherein the interior space of the hydrocyclone converges towards the outlet, delivering the suspension to the inlet of the hydrocyclone, draining off the suspension enriched with the cells or the other material from the outlet;
• and guiding the enriched suspension from or through the outlet by a tube, which is formed so that at its end opposite to the outlet, the kinetic energy of the enriched suspension is small enough to avoid any damage to the cells or the material of the suspension when it exits the tube.

A method for separating eukaryotic cells, procaryotic cells, or other biological material which is sensitive to high shearing forces from a suspension, the method comprising the steps of:

• providing at least one hydrocyclone with an inlet and an outlet, the outlet being essentially opposite to the inlet wherein the interior space of the hydrocyclone converges towards the outlet, delivering the suspension to the inlet of the hydrocyclone, draining off the suspension enriched with the cells or the other material from the outlet;
• and guiding the enriched suspension from or through the outlet by a tube so that at its end opposite to the outlet, the flow means transports the enriched suspension below the upper level of a fluid within a bioreactor.

A method for separating eukaryotic cells, procaryotic cells, or other biological material which is sensitive to high shearing forces from a suspension, the method comprising the steps of:

• providing at least one hydrocyclone with an inlet and an outlet, the outlet being essentially opposite to the inlet wherein the interior space of the hydrocyclone converges towards the outlet, delivering the suspension to the inlet of the hydrocyclone, draining off the suspension enriched with the cells or the other material from the outlet;
• and guiding the enriched suspension from or through the outlet directly below the upper level of a fluid within a bioreactor.

An apparatus for separating eukaryotic cells, procaryotic cells, or other biological material which is sensitive to high shearing forces from a suspension, the apparatus comprising:

• at least one hydrocyclone with an inlet and an outlet, the outlet being essentially opposite to the inlet wherein the interior space of the hydrocyclone converges towards the outlet, an inlet of the hydrocyclone for delivering the suspension into the hydrocyclone, an outlet to drain off the suspension being enriched with cells or the other material, and a tube for guiding the enriched suspension from or through the outlet which is oriented in an essentially acute angle towards the longitudinal axis of the outlet, or the longitudinal axis of the hydrocyclone so that the enriched suspension at least close to the outlet has an essential tangential flow within the tube.

An apparatus for separating eukaryotic cells, procaryotic cells, or other biological material which is sensitive to high shearing forces from a suspension, the apparatus comprising:

• at least one hydrocyclone with an inlet and an outlet, the outlet being essentially opposite to the inlet wherein the interior space of the hydrocyclone converges towards the outlet, an inlet of the hydrocyclone for delivering the suspension into the hydrocyclone, an outlet to drain off the suspension being enriched with cells or the other material;
• and a tube for guiding the eruiched suspension from or through the outlet, the tube being formed so that at its end opposite to the outlet the kinetic energy of the enriched suspension is small enough to avoid any damage to the cells or the material of the suspension when it is emitted from the tube.

An apparatus for separating eukaryotic cells, procaryotic cells, or other biological material which is sensitive to high shearing forces from a suspension, the apparatus comprising:

• at least one hydrocyclone with an inlet and an outlet, the outlet being essentially opposite to the inlet wherein the interior space of the hydrocyclone converges towards the outlet, an inlet of the hydrocyclone for delivering the suspension into the hydrocyclone, an outlet to drain off the suspension being enriched with cells or the other material;
• and a tube for guiding the enriched suspension from or through the outlet, the flow means being formed so that at its end opposite to the outlet, the tube transports the enriched suspension below the upper level of a fluid within a bioreactor.

An apparatus for separating eukaryotic cells, procaryotic cells, or other biological material which is sensitive to high shearing forces from a suspension, the apparatus comprising:

• at least one hydrocyclone with an inlet and an outlet, the outlet being essentially opposite to the inlet wherein the interior space of the hydrocyclone converges towards the outlet, an inlet of the hydrocyclone for delivering the suspension into the hydrocyclone, an outlet to drain off the suspension being enriched with cells or the other material, guiding the enriched suspension from or through the outlet directly below the upper level of a fluid within a bioreactor.