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AssigneeThe Brigham and Woman's Hospital, Inc.
InventorsLaurie E. Comstock, Katja G. Weinacht, Michael J. Coyne, Dennis L. Kasper, Arthur O. Tzianabos
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The present invention is directed to methods for producing and selecting new mutant strains of B. fragilis that constitutively express a particular capsular polysaccharide or only selected capsular polysaccharides; compositions directed to the new mutant strains of B. fragilis that constitutively express a particular capsular polysaccharide or only selected capsular polysaccharides; improved methods for purification of individual capsular polysaccharides; and compositions directed to new res02 and inv19 genes and their gene products. Significantly, the present invention provides methods and compositions for overexpressing and purifying immunomodulatory capsular polysaccharide A (PSA) in high yield.
An isolated Bacteroides fragilis bacterial cell producing a native capsular polysaccharide A (PSA), and having a native promoter that controls expression of native biosynthetic genes of PSA and native promoters that control expression of native biosynthetic genes of native capsular polysaccharides PSB, PSD, PSE, PSF, PSG and PSH,
An isolated Bacteroides fragilis bacterial cell producing a first native capsular polysaccharide selected from the group consisting of:
Dependencies are inferred from the claim text.