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US 7,803,598 B2Granted
Cyclodextrin glycosyltransferase (CGTase) polypeptides with modified hydrolysis activity
AssigneeDanisco A/S
InventorsKarsten M. Kragh, Hans Leemhuis, Lubbert Dijkhuizen, Bauke W. Dijkstra
Life of this patent
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Abstract
An enzyme is described which enzyme is derived from family 13 of α-amylases. The enzyme variant is obtainable by modifying a CGTase or a maltogenic α-amylase. The enzyme is useful in preparing a food or a food product such as bakery products.
Claims5
An isolated and/or purified cyclodextrin glycosyltransferase (CGTase) polypeptide with increased hydrolase activity, comprising the amino acid sequence as set forth in SEQ ID NO:
- 1 or 3, wherein
- (a) said CGTase polypeptide has a variation at position 230, A230V, and
- (b) said CGTase polypeptide optionally has amino acid substitutions selected from the group consisting of F21L, A245T, A357T, N94S, R47W, R47L, R47Q, K232L, E264A, Y633A, W616A, W662A, L600A, A231V, F260E, and F260I.
Dependencies are inferred from the claim text.