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AssigneeThe Rockefeller University
InventorsOlga Yurieva, John Kuriyan, Michael E. O'Donnell, David Jeruzalmi
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A DNA Polymerase has been identified in a thermophile that functions as a chromosomal replicase. The specific enzyme is a holoenzyme III that has been identified in Thermus thermophilus, and corresponds to Polymerase III in E. coli. The genes and the polypeptides corresponding to T.th. γ, τ, ε, α and β subunits that they encode are disclosed, as are probes, vectors, methods of preparation and the methods of use. The enzymes of the present invention and their components are particularly well suited for use in procedures for the preparation of DNA, such as PCR, because of the speed and accuracy that they are able to achieve.
An isolated polynucleotide encoding an ε subunit of a DNA polymerase III-type enzyme of a Thermus bacterium, the encoded ε subunit possessing DNA polymerase-associated 3′-5′ exonuclease activity, wherein said isolated polynucleotide is at least 95 percent identical to the nucleotide sequence of SEQ ID NO:
An isolated polynucleotide encoding an ε subunit of a DNA polymerase III-type enzyme of a thermophilic bacterium, wherein the polynucleotide comprises a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:
Dependencies are inferred from the claim text.