Heat-stable carbonic anhydrases and their use
AssigneeNovozymes A/S
InventorsMartin Borchert, Paria Saunders
Life of this patent
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Abstract
The present invention relates to use of heat-stable carbonic anhydrase in CO2 extraction, e.g., from flue gas, natural gas or biogas. Furthermore, the invention relates to isolated polypeptides having carbonic anhydrase activity at elevated temperatures and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides.
Claims29
A method of using a heat-stable alpha-class carbonic anhydrase comprising:
- contacting a carbon dioxide-containing medium with a heat stable carbonic anhydrase, wherein carbon dioxide is extracted from the carbon dioxide-containing medium, wherein the alpha-class carbonic anhydrase is an isolated polypeptide selected from the group consisting of:
- (a) a polypeptide having an amino acid sequence which has at least 96% identity with the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 4, or at least 95% identity with the amino acid sequence of SEQ ID NO:
- 6, or at least 98% identity with the amino acid sequence of SEQ ID NO:
- 8, or at least 91% identity with the amino acid sequence of SEQ ID NO:
- 10, or at least 99% identity with the amino acid sequence of SEQ ID NO:
- 12;
- (b) a polypeptide selected from the group consisting of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 10 and SEQ ID NO:
- 12, and
- (c) a fragment of (a) or (b) having carbonic anhydrase activity.
An isolated polypeptide having carbonic anhydrase activity at elevated temperatures, selected from the group consisting of:
- a polypeptide having an amino acid sequence which has at least 96% identity with the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 4, or at least 95% identity with the amino acid sequence of SEQ ID NO:
- 6, or at least 98% identity with the amino acid sequence of SEQ ID NO:
- 8, or at least 91% identity with the amino acid sequence of SEQ ID NO:
- 10, or at least 99% identity with the amino acid sequence of SEQ ID NO:
- 12 or enzymatically active fragment thereof.
An isolated polypeptide having carbonic anhydrase activity at elevated temperatures having at least 97% sequence identity with SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
An isolated polypeptide having carbonic anhydrase activity at elevated temperatures having at least 96% sequence identity with SEQ ID NO:
- 6.
An isolated polypeptide having carbonic anhydrase activity at elevated temperatures having at least 96% sequence identity with SEQ ID NO:
- 10.
An isolated polynucleotide encoding a polypeptide having carbonic anhydrase activity at elevated temperatures, selected from the group consisting of:
- a polypeptide having an amino acid sequence which has at least 96% identity with the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 4, or at least 95% identity with the amino acid sequence of SEQ ID NO:
- 6, or at least 98% identity with the amino acid sequence of SEQ ID NO:
- 8, or at least 91% identity with the amino acid sequence of SEQ ID NO:
- 10, or at least 99% identity with the amino acid sequence of SEQ ID NO:
- 12 or enzymatically active fragment thereof.
An isolated polypeptide having carbonic anhydrase activity at elevated temperatures, selected from the group consisting of:
- a polypeptide having an amino acid sequence which has at least 96% identity with the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 4, or at least 95% identity with the amino acid sequence of SEQ ID NO:
- 6, or at least 98% identity with the amino acid sequence of SEQ ID NO:
- 8, or at least 91% identity with the amino acid sequence of SEQ ID NO:
- 10, or at least 99% identity with the amino acid sequence of SEQ ID NO:
- 12.
Dependencies are inferred from the claim text.