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US 7,803,571 B2Granted
Method for identifying IRS inhibitors or agonists
AssigneeSanfoi-Aventis Deutschland GmbH
InventorsNorbert Tennagels, Jurgen Eckel, Sabine Metzger, Mark Sommerfeld
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Abstract
The present invention relates to a method for the identification of an IRS protein kinase inhibitor, comprising the steps of a) bringing into contact PKC-ζ with at least one IRS peptide comprising at least one PKC-ζ-Ser-phosphorylation site in the presence of at least one possible inhibitor, and b) measuring the phosphorylation of the PKC-ζ-Ser-phosphorylation site.
Claims6
A method for identifying an IRS protein kinase inhibitor, the method comprising the steps of
- a) contacting, in the presence of at least one possible inhibitor, PKC-ζ with at least one IRS peptide comprising amino acid sequence of SEQ ID NO:17 comprising at least one PKC-ζ-Ser-phosphorylation site, and
- b) measuring the phosphorylation of a PKC-ζ-Ser-phosphorylation site at a residue selected from the group consisting of residues 498, 570, and 612, wherein phosphorylation at one or more of residues 498, 570 or 612 inhibits insulin-stimulated tyrosine phosphorylation of the IRS peptide or inhibits interaction of p85α regulatory subunit of phosphatidylinositol 3-kinase with the IRS peptide.
Dependencies are inferred from the claim text.