Methods and compositions for detecting trichomonas in a sample contacted with fixative
AssigneeBoard of Regents, The University of Texas System
InventorsJohn F. Alderete, Te-Hung Chang
Life of this patent
Estimating the term…
Abstract
The present invention provides methods and compositions for detecting a Trichomonas antigen in a fixed sample and for diagnosing Trichomonas infection by detecting a Trichomonas antigen in a fixed sample.
Claims12
A method of detecting a Trichomonas antigen in a sample from a human subject, wherein the sample from the subject has been contacted with fixative, comprising:
- a) contacting the sample with an antibody that specifically binds a fixed Trichomonas alpha actinin antigen under conditions whereby an antigen/antibody complex can form;
- and b) detecting the formation of an antigen/antibody complex, thereby detecting the Trichomonas antigen in the sample, wherein the antibody is a monoclonal antibody produced from the hybridoma cell line HA423, deposited with the American Type Culture Collection under Accession No. PTA-10802.
A method of detecting a Trichomonas antigen in a sample from a human subject, comprising:
- a) contacting the sample from the subject with a fixative;
- b) contacting the sample of (a) with an antibody that specifically binds a fixed Trichomonas alpha actinin antigen under conditions whereby an antigen/antibody complex can form;
- and c) detecting the formation of an antigen/antibody complex, thereby detecting the Trichomonas antigen in the sample, wherein the antibody is a monoclonal antibody produced from the hybridoma cell line HA423, deposited with the American Type Culture Collection under Accession No. PTA-10802.
A method of diagnosing Trichomonas infection in a human subject, comprising:
- a) contacting a sample from the subject, wherein the sample has been contacted with a fixative, with an antibody that specifically binds a fixed Trichomonas alpha actinin antigen under conditions whereby an antigen/antibody complex can form;
- and b) detecting formation of an antigen/antibody complex, thereby diagnosing a Trichomonas infection in the subject, wherein the antibody is a monoclonal antibody produced from the hybridoma cell line HA423, deposited with the American Type Culture Collection under Accession No. PTA-10802.
A method of diagnosing Trichomonas infection in a human subject, comprising:
- a) contacting a sample from the subject with a fixative;
- b) contacting the sample of (a) with an antibody that specifically binds a fixed Trichomonas alpha actinin antigen under conditions whereby an antigen/antibody complex can form;
- and c) detecting formation of an antigen/antibody complex, thereby diagnosing Trichomonas infection in the subject, wherein the antibody is a monoclonal antibody produced from the hybridoma cell line HA423, deposited with the American Type Culture Collection under Accession No. PTA-10802.
Dependencies are inferred from the claim text.