Method for screening the receptor-ligand binding in live cell
AssigneesShanghai Institutes for Biological Sciences, Cas, Peking University
InventorsZhuan Zhou, Hoping Cheng
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Abstract
The invention relates to methods and system for detecting ligand binding to membrane receptors and endocytosis. A method for detecting ligand binding to a membrane receptor includes the steps of: incubating the cell with a dye and a ligand; and detecting dye-containing endocytic vesicles in the cell. A system for screening ligand binding to membrane receptors includes an automatic liquid handling device; an x-y stage for positioning a plate; a microscope configured to image endocytic vesicles of a cell; a software for automatic analysis of FM spots (endocytic vesicles); and a control unit configured to control the movement of the x-y stage and the microscope.
Claims13
A method for detecting ligand binding to a membrane receptor, comprising the steps of:
- incubating a cell with a mixture of a dye and a ligand;
- and
- detecting dye-containing endocytic vesicles in the cell, wherein detection of the dye-containing endocytic vesicles at a level higher than a background level indicates said ligand binding to said membrane receptor, wherein the background level is obtained by incubating the cell with the dye in the absence of the ligand.
Dependencies are inferred from the claim text.