Back to results
US 7,803,560 B2Granted
Methods of screening for antagonists of mammalian monocyte chemoattractant protein receptors
AssigneeThe Regents of the University of California
InventorsIsrael F. Charo, Shaun R. Coughlin
Life of this patent
Estimating the term…
Abstract
Novel human chemokine receptors, MCP-1RA and MCP-1RB, and processes for producing them are disclosed. The receptors, which are alternately spliced versions of MCP-1 receptor protein may be used in an assay to identify antagonists of MCP-1 which are therapeutically useful in the treatment of atherosclerosis and other diseases characterized by monocytic infiltrates.
Claims9
A method to identify an antagonist of MCP-1R, comprising:
- (a) contacting an isolated MCP-1R polypeptide with a candidate antagonist, wherein the MCP-1R polypeptide comprises an amino acid sequence at least 95% homologous to an extracellular domain of SEQ ID NO:
- 2 or of SEQ ID NO:
- 4, and
- (b) comparing the binding of MCP-1 to the MCP-1R polypeptide in the presence of the candidate antagonist to the binding of MCP-1 to the MCP-1R polypeptide in its absence;
- wherein a candidate antagonist which causes a decrease in the binding of MCP-1 to the MCP-1R polypeptide is identified as an antagonist of MCP-1R.
2 more elements
Dependencies are inferred from the claim text.