Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer

A method of isolating and purifying nucleic acids using an immobilized polyethylene glycol (PEG)-containing hydrogel, the method comprising:

• immobilizing a polyethylene glycol (PEG)-containing hydrogel on a substrate;
• adding a mixed sample solution containing a salt and nucleic acids to the PEG-containing hydrogel immobilized substrate to bind the nucleic acids to the PEG-containing hydrogel;
• washing the nucleic acid-bound PEG-containing hydrogel;
• eluting the nucleic acids from the nucleic acid-bound PEG-containing hydrogel using an elution solvent, and
• recovering the eluted nucleic acids,
• wherein the PEG-containing hydrogel is a reaction product of PEG and a polymer represented by formula I below,
• wherein an incorporation ratio of the PEG is 1 to 20 mole % based on carboxyl groups formed from hydrolysis of the polymer of formula I and the PEG is bound to the carboxyl groups of the hydrolyzed polymer via an ester bond:
• wherein,
• R1 through R6 are each independently a hydrogen atom, a halogen atom, a hydroxy group, a substituted or unsubstituted alkyl group of 1-20 carbon atoms, a substituted or unsubstituted alkoxy group of 1-20 carbon atoms, a substituted or unsubstituted alkenyl group of 1-20 carbon atoms, a substituted or unsubstituted aryl group of 6-30 carbon atoms, a substituted or unsubstituted aryloxy group of 6-30 carbon atoms, a substituted or unsubstituted heteroaryl group of 2-30 carbon atoms, or a substituted or unsubstituted heteroaryloxy group of 2-30 carbon atoms;
• and n is an integer from 2 to 100,000.