Chlamydia PMP proteins, gene sequences and uses thereof
AssigneeEmergent Product Development Gaithersburg Inc.
InventorW. James Jackson
Life of this patent
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Abstract
The invention discloses the Chlamydia PMPE and PMPI polypeptide, polypeptides derived therefrom, (PMP-derived polypeptides), nucleotide sequences encoding said polypeptides, antibodies that specifically bind the PMP polypeptides and PMP-derived polypeptides and T-cells specific for PMP polypeptides and PMP-derived polypeptides. Also disclosed are prophylactic and therapeutic compositions, including immunogenic compositions, e.g., vaccines, comprising PMP polypeptides or PMP-derived polypeptides or antibodies thereto. The invention additionally discloses methods of inducing in animals an immune response to Chlamydia cells, Chlamydia elementary bodies, and/or cells expressing Chlamydial proteins, e.g., cell infected with Chlamydia.
Claims40
An isolated polypeptide comprising the amino acid sequence selected from the group consisting of:
- (a) amino acids 32-965 of SEQ ID NO:
- 2;
- (b) SEQ ID NO:
- 2;
- (c) amino acids 625-700 of SEQ ID NO:
- 2;
- (d) amino acids 590-625 of SEQ ID NO:
- 2;
- (e) amino acids 1-500 of SEQ ID NO:
- 2;
- (f) the amino acid sequence encoded by the Chlamydia insert in Escherichia coli M15 assigned as ATCC PTA No. 2462;
- and
- (g) a combination of two or more amino acid sequences of (a)-(f).
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
- 4 or the amino acid sequence encoded by a Chlamydia insert in E. coli TOP10 assigned as ATCC PTA No. 2461.
An isolated polypeptide consisting of the amino acid sequence selected from the group consisting of:
- amino acids 32-965 of SEQ ID NO:
- 2;
- SEQ ID NO:
- 2;
- the amino acid sequence encoded by the Chlamydia insert in E. coli M15 assigned as ATCC PTA No. 2462;
- amino acids 15-56 of SEQ ID NO:
- 2;
- amino acids 15-121 of SEQ ID NO:
- 2;
- amino acids 45-125 of SEQ ID NO:
- 2;
- amino acids 125-190 of SEQ ID NO:
- 2;
- amino acids 195-261 of SEQ ID NO:
- 2;
- amino acids 275-366 of SEQ ID NO:
- 2;
- amino acids 375-440 of SEQ ID NO:
- 2;
- amino acids 440-490 of SEQ ID NO:
- 2;
- amino acids 525-590 of SEQ ID NO:
- 2;
- amino acids 590-625 of SEQ ID NO:
- 2;
- amino acids 615-650 of SEQ ID NO:
- 2;
- amino acids 625-700 of SEQ ID NO:
- 2;
- amino acids 725-800 of SEQ ID NO:
- 2;
- amino acids 755-775 of SEQ ID NO:
- 2;
- amino acids 785-845 of SEQ ID NO:
- 2;
- amino acids 815-865 of SEQ ID NO:
- 2;
- amino acids 1-31 of SEQ ID NO:
- 2;
- and amino acids 1-500 of SEQ ID NO:
- 2.
An isolated polypeptide consisting of the amino acid sequence selected from the group consisting of:
- SEQ ID NO:
- 4, amino acids 13-40 of SEQ ID NO:
- 4;
- amino acids 70-110 of SEQ ID NO:
- 4;
- amino acids 150-225 of SEQ ID NO:
- 4;
- amino acids 250-290 of SEQ ID NO:
- 4;
- amino acids 370-455 of SEQ ID NO:
- 4;
- amino acids 400-455 of SEQ ID NO:
- 4;
- amino acids 470-520 of SEQ ID NO:
- 4;
- amino acids 615-670 of SEQ ID NO:
- 4;
- amino acids 710-775 of SEQ ID NO:
- 4;
- amino acids 765-825 of SEQ ID NO:
- 4;
- amino acids 830-860 of SEQ ID NO:
- 4;
- and amino acids 1-500 of SEQ ID NO:
- 4.
An isolated polypeptide comprising the amino acid sequence selected from the group consisting of:
- amino acids 32-965 of SEQ ID NO:
- 2;
- SEQ ID NO:
- 2;
- the amino acid sequence encoded by the Chlamydia insert in E. coli M15 assigned as ATCC PTA No. 2462;
- amino acids 15-56 of SEQ ID NO:
- 2;
- amino acids 15-121 of SEQ ID NO:
- 2;
- amino acids 45-125 of SEQ ID NO:
- 2;
- amino acids 125-190 of SEQ ID NO:
- 2;
- amino acids 195-261 of SEQ ID NO:
- 2;
- amino acids 275-366 of SEQ ID NO:
- 2;
- amino acids 375-440 of SEQ ID NO:
- 2;
- amino acids 440-490 of SEQ ID NO:
- 2;
- amino acids 525-590 of SEQ ID NO:
- 2;
- amino acids 590-625 of SEQ ID NO:
- 2;
- amino acids 615-650 of SEQ ID NO:
- 2;
- amino acids 625-700 of SEQ ID NO:
- 2;
- amino acids 725-800 of SEQ ID NO:
- 2;
- amino acids 755-775 of SEQ ID NO:
- 2;
- amino acids 785-845 of SEQ ID NO:
- 2;
- amino acids 815-865 of SEQ ID NO:
- 2;
- amino acids 1-31 of SEQ ID NO:
- 2;
- and amino acids 1-500 of SEQ ID NO:
- 2, wherein said isolated polypeptide further comprises a heterologous polypeptide.
An isolated polypeptide comprising the amino acid sequence selected from the group consisting of:
- SEQ ID NO:
- 4, amino acids 13-40 of SEQ ID NO:
- 4;
- amino acids 70-110 of SEQ ID NO:
- 4;
- amino acids 150-225 of SEQ ID NO:
- 4;
- amino acids 250-290 of SEQ ID NO:
- 4;
- amino acids 370-455 of SEQ ID NO:
- 4;
- amino acids 400-455 of SEQ ID NO:
- 4;
- amino acids 470-520 of SEQ ID NO:
- 4;
- amino acids 615-670 of SEQ ID NO:
- 4;
- amino acids 710-775 of SEQ ID NO:
- 4;
- amino acids 765-825 of SEQ ID NO:
- 4;
- amino acids 830-860 of SEQ ID NO:
- 4;
- and amino acids 1-500 of SEQ ID NO:
- 4, wherein said isolated polypeptide further comprises a heterologous polypeptide.
Dependencies are inferred from the claim text.