Poly-gamma-glutamic conjugates for eliciting immune responses directed against bacilli
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsRachel Schneerson, Stephen Leppla, John B. Robbins, Joseph Shiloach, Joanna Kubler-Kielb, Darrell Liu, Fathy Majadly
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Abstract
Immunogenic compositions and methods for eliciting an immune response against B. anthracis and other bacilli are provided that include immunogenic conjugates of a poly-γ-glutamic acid (γPGA) polypeptide of B. anthracis, or of another Bacillus that expresses a γPGA polypeptide. The γPGA conjugates elicit an effective immune response against B. anthracis, or against another Bacillus, in mammalian hosts to which the conjugates are administered.
Claims42
An immunogenic conjugate comprising a synthetic homopolymer of poly-γ-glutamic acid (γPGA) polypeptide covalently linked to a carrier, wherein the conjugate elicits an immune response against poly-γ-glutamic acid (γPGA) polypeptide in a subject.
An immunogenic conjugate comprising a Bacillus poly-γ-glutamic acid (γPGA) polypeptide covalently linked to a carrier, wherein the carrier is selected from the group consisting of:
- (a) recombinant B. anthracis protective antigen, (b) recombinant P. aeruginosa exotoxin A, (c) tetanus toxoid, (d) diphtheria toxoid, (e) pertussis toxoid, (f) C. perfringens toxoid, (g) hepatitis B surface antigen, (h) hepatitis B core antigen, (i) keyhole limpet hemocyanin, (j) horseshoe crab hemocyanin, (k) edestin, (l) mammalian serum albumins, and (m) combinations thereof, and wherein the conjugate elicits an immune response against Bacillus poly-γ-glutamic acid (γPGA) polypeptide in a subject.
An immunogenic conjugate comprising poly-γ-glutamic acid (γPGA) covalently linked to a carrier, wherein the conjugate elicits an immune response against poly-γ-glutamic acid (γPGA) in a subject, and the conjugate has a density of γPGA chains to carrier molecule of at least about 5:1.
Dependencies are inferred from the claim text.