US 7,799,527 B2Granted

Systems and methods for automated quality control of polymer synthesis

AssigneeIllumina, Inc.

InventorsNels A. Olson, Bahram Ghaffarzadeh Kermani, Hongji Ren, Theo Kotseroglou

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Abstract

The invention provides systems and methods for evaluating synthetic reactions such as polymer synthesis reactions. Evaluation of a synthetic reaction product can be based on a quantitative measure of synthesis such as coupling efficiency or yield, and various parameters determined from a separation record obtained for the synthetic reaction product. The invention further provides methods for evaluating a separation method based on various parameters determined from a separation record. The invention can be used to select an appropriate treatment for a synthetic reaction product such as a separation treatment or treatments related to further processing for the desired product. The methods are particularly useful for automated selection of treatments for synthetic reaction products.

Claims19

Dependent claims

A method of selecting at least one treatment for a desired synthesized polymer species, comprising the automated steps of:

(a) providing a separation record for a polymer product comprising a desired synthesized polymer species, wherein said separation record comprises a baseline and at least one peak representing said desired synthesized polymer species;
(b) producing a baseline-evaluated separation record by evaluating said baseline and optionally adjusting said baseline;
(c) identifying, in said baseline-evaluated separation record, at least one peak representing said desired synthesized polymer species;
(d) determining a quality metric for said desired synthesized polymer species based on a plurality of parameters of said baseline-evaluated separation record, said parameters comprising signal-to-noise ratio of said at least one peak of said baseline-evaluated separation record and retention of said at least one peak of said baseline-evaluated separation record;
(e) determining a coupling efficiency of the synthesis reaction of said desired synthesized polymer species;
and
(f) evaluating the quality of said desired synthesized polymer species based on said quality metric and said coupling efficiency, and selecting at least one treatment for said desired synthesized polymer species from a plurality of alternative treatments based on the value of said quality metric or said coupling efficiency.

4 more elements

A method of selecting at least one treatment for a plurality of different desired synthesized polymer species, comprising the automated steps of:

(a) providing separation records for polymer products comprising a plurality of different desired synthesized polymer species, wherein each of said separation records comprises a baseline and at least one peak representing one of said different desired synthesized polymer species;
(b) producing a plurality of baseline-evaluated separation records by evaluating each said baseline of each of said separation records and optionally adjusting said baseline;
(c) identifying, in each of said baseline-evaluated separation records, at least one peak representing one of said different desired synthesized polymer species;
(d) determining a quality metric for each of said different desired synthesized polymer species based on a plurality of parameters of each of said baseline-evaluated separation records, said parameters comprising signal-to-noise ratio of said at least one peak of each of said baseline evaluated separation records, and retention of said at least one peak of each of said baseline evaluated separation records;
(e) determining a coupling efficiency of the synthesis reaction of each of said different desired synthesized polymer species;
and
(f) evaluating the quality of each of said different desired synthesized polymer species based on said quality metric and said coupling efficiency, and selecting at least one treatment for each of said different desired synthesized polymer species from a plurality of alternative treatments based on the value of said quality metric or said coupling efficiency.

4 more elements

Dependencies are inferred from the claim text.