Loading patent…
AssigneeThe Commonwealth of Australia Commonwealth Scientific and Industrial Research Organisation
InventorsZhongyi Li, Matthew Kennedy Morell, Sadequr Rahman
Estimating the term…
Rice having reduced levels of starch branching enzymes produce grain having a high relative amylose content in the endosperm. The rice grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway and may be transgenic or nontransgenic. 1 GCCACCGACA TCCGCCGCAA TGCTGTGTCT CACCTCCTCT TCCTCCTCCG CGCCCGCTCC 61 GCTCCTTCCC TCTCTCGCTG ATCGACCGAG CCCGGGAATC GCGGGCGGGG GTGGCAATGT 121 TCGCCTGAGC GTGGTTTCTT CGCCGCGCCG GTCGTGGCCT GGAAAGGTCA AGACCAATTT 181 CTCAGTTCCT GCGACTGCGC GAAAAAACAA AACCATGGTG ACTGTTGTGG AGGAGGTCGA 241 CCACCTTCCT ATATATGATC TGGACCCTAA GTTGGAGGAA TTCAAGGATC ACTTCAACTA 301 TAGGATAAAA AGATACCTCG ACCAGAAATG CCTGATTGAA AAACATGAGG GGGGCCTTGA 361 AGAATTTTCT AAAGGCTATT TGAAGTTTGG GATTAATACA GTTGATGGTG CCACAATATA 421 TCGTGAATGG GCGCCTGCTG CACAAGAAGC ACAGCTCATT GGTGAGTTCA ATAACTGGAA 481 TGGTGCAAAA CACAAGATGG AGAAGGATAA ATTTGGCATT TGGTCAATCA AGATTTCACA 541 TGTCAATGGG AAGCCTGCCA TCCCTCACAA TTCCAAGGTT AAATTTCGCT TTAGGCATGG 601 GGGTGGAGCA TGGGTTGATC GTATTCCCGC ATGGATTCGT TATGCAACTT TTGATGCCTC 661 TAAATTTGGA GCTCCATATG ATGGTGTACA CTGGGATCCT CCAGCCTGTG AAAGGTACGT 721 GTTTAAGCAT CCTCGACCTC CAAAACCTGA TGCTCCACGC ATCTATGAGG CTCATGTGGG 781 GATGAGTGGT GAAGAGCCAG AAGTAAGCAC ATACAGAGAA TTTGCAGACA ATGTGTTACC 841 ACGCATACGG GCAAATAACT ACAACACAGT TCAGTTAATG GCAATCATGG AACATTCCTA 901 CTATGCTTCT TTTGGGTATC ACGTGACAAA TTTTTTCGCA GTCAGCAGCA GATCAGGAAC 961 ACCAGAGGAT CTGAAATATC TTGTTGACAA GGCACATAGT TTAGGATTAC GAGTTCTGAT 1021 GGATGTTGTC CATAGCCATG CGAGTAATAA TGTGACCGAT GGTCTAAATG GCTATGACGT 1081 TGGACAAAAC ACTCATGAGT CTTATTTTCA TACAGGAGAT AGGGGCTACC ATAAACTCTG 1141 GGATAGTCGT CTGTTCAACT ATGCCAATTG GGAGGTCTTA AGATTTCTTC TTTCTAATTT 1201 GAGATATTGG ATGGACGAAT TCATGTTTGA TGGCTTCCGA TTTGATGGGG TTACATCAAT 1261 GCTATACCAT CACCATGGTA TCAATAAGGG ATTTACTGGA AACTACAAGG AGTATTTCAG 1321 TTTGGATACC GATGTGGATG CAATTGTTTA CATGATGCTC GCAAACCATT TAATGCATAA 1381 ACTCTTGCCG GAAGCAACTA TTGTTGCTGA AGATGTTTCG GGCATGCCAG TGCTTTGTCG 1441 GCCAGTTGAT GAAGGTGGAG TAGGGTTTGA CTTCCGCCTG GCAATGGCCA TTCCTGATAG 1501 ATGGATTGAC TACCTGAAGA ACAAAGAGGA CCGCAAATGG TCAATGAGTG AAATAGTGCA 1561 AACTTTGACT AACAGGAGAT ATACAGAAAA ATGCATTGCC TATGCCGAGA GCCATGATCA 1621 GTCCATTGTT GGTGACAAGA CTATAGCATT TCTCTTGATG GACAAGGAAA TGTACACTGG 1681 CATGTCAGAC TTGCAGCCTG CTTCACCTAC CATCAACCGT GGCATTGCAC TCCAAAAGAT 1741 GATTCACTTC ATTACGATGG CCCTTGGAGG TGATGGCTAC TTAAATTTTA TGGGCAATGA 1801 GTTTGGCCAT CCAGAATGGA TTGACTTTCC AAGAGAAGGC AACAACTGGA GCTATGATAA 1861 ATGCAGACGT CAGTGGAGCC TTGTCGACAC TGATCACCTT CGATACAAGT ATATGAATGC 1921 ATTTGATCAA GCAATGAATG CACTCGAGGA GGAATTTTCC TTCCTGTCAT CATCAAAGCA 1981 GATTGTTAGC GACATGAACG AGAAAGATAA GGTTATTGTC TTTGAACGTG GAGATTTGGT 2041 TTTTGTTTTC AATTTTCATC CCAACAAAAC TTATAAGGGT TACAAAGTCG GATGTGACTT 2101 GCCCGGGAAG TACAGAGTAG CTCTGGACTG TGATGCTTTG GTCTTTGGTG GCCATGGAAG 2161 AGTTGGCCAT GATGTGGATC ACTTCACGTC TCCCGAGGGA ATGCCAGGAG TACCAGAAAC 2221 AAATTTCAAC AACCGCCCTA ACTCATTCAA AGTCCTTTCC CCGCCCCGTA CCTGTGTGGC 2281 TTACTATCGC GTTGATGAAG ATCGTGAAGA GCTCAGGAGG GGTGGAGCAG TTGCTTCTGG 2341 AAAGATTGTT ACAGAGTATA TCGATGTTGA AGCAACAAGT GGGGAGACTA TCTCTGGTGG 2401 CTGGAAGGGC TCCGAGAAGG ACGATTGTGG CAAGAAAGGG ATGAAGTTTG TGTTTCGGTC 2461 TTCTGACGAA GACTGCAAAT GAAGCATCAG ATTTCTTGAT CAGGAGCAAC TGTTGGTGCC 2521 CTTGTAATCT GGAGATCCTG GCTTGCCTTG GACTTGGTTG TGGTTCTTTA GCAGTTGCTA 2581 TGTACCTATC TATGATATGA ACTTTATGTA TAGTTCGCCT TAAAGAAAGA ATAAGCAGTG 2641 ATGATGTGGC CTTAAACCTG AGCTGCACAA GCCTAATGTA AAAATAAAGT TTCAGGCTTT 2701 CATCCAGAAT AAAACAGCTG TTCATTTACC ATCTCAAAA
Grain obtained from a rice plant, comprising (i) starch, (ii) an exogenous nucleic acid molecule comprising a sequence which is the same as, or fully complementary to, a nucleotide sequence of at least 50 contiguous nucleotides of the starch branching enzyme IIa (SBEIIa) gene coding region whose sequence is set forth in SEQ ID NO:2 which inhibits the expression of a rice SBEIIa gene, (iii) a reduced level of SBEIIa protein and, (iv) a reduced level of starch branching enzyme IIb (SBEIIb) protein in the rice grain, the reduced level of SBEIIa protein and of SBEIIb protein being relative to rice grain of an Indica variety,
A rice plant comprising i) an exogenous nucleic acid molecule comprising a sequence which is the same as, or fully complementary to, a nucleotide sequence of at least 50 contiguous nucleotides of the starch branching enzyme IIa (SBEIIa) gene coding region whose sequence is set forth in SEQ ID NO:2 which inhibits the expression of a rice SBEIIa gene, (ii) a reduced level of SBEIIa protein, (iii) a reduced level of starch branching enzyme IIb (SBEIIb) protein in the rice plant, the reduced level of SBEIIa protein and of SBEIIb protein being relative to rice plant of an Indica variety, and (iv) rice grain
A method of producing a rice plant capable of producing grain, the grain having starch comprising at least 40% amylose, comprising the steps of
An isolated nucleic acid molecule comprising a sequence which is the same as, or fully complementary to, a nucleotide sequence of at least 50 contiguous nucleotides set forth in SEQ ID NO:2 which, which encodes an inhibitor of rice SBEIIa and an inhibitor of rice SBEIIb, which may be the same or different.
Dependencies are inferred from the claim text.