Vector expressing n-deacetylase/n-sulfotransferase 2
AssigneesRiken, Seikagaku Corporation
InventorsJun-ichi Aikawa, Masafumi Tsujimoto, Takuji Kaneko
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Abstract
An object of the present invention is to provide an expression vector that allows for stable production of N-deacetylase/N-sulfotransferase 2 in large amounts and a process for production of N-deacetylase/N-sulfotransferase 2 using the same. The present invention provides a recombinant baculovirus expression vector obtained by incorporating into baculovirus DNA, a DNA fragment having lobster L21 DNA, DNA encoding gp67 signal peptide and DNA encoding the 79th to 883rd amino acids of human N-deacetylase/N-sulfotransferase 2 in this order in the 5′ to 3′ direction.
Claims9
A vector which comprises a signal peptide-encoding DNA that functions in a baculovirus expression vector, wherein the signal peptide is from baculovirus gp67, from silkworm bombyxin signal peptide, or bee melittin signal peptide;
- and
- a DNA ligated downstream of the signal peptide-encoding DNA, wherein the DNA encodes amino acids consisting of 79 to 883 of SEQ ID NO:2.
A recombinant baculovirus expression vector comprising
- baculovirus DNA,
- a DNA encoding a signal peptide positioned 3′ to the baculovirus DNA, wherein the signal peptide functions in a baculovirus expression vector and is from baculovirus gp67, from silkworm bombyxin signal peptide, or bee melittin signal peptide;
- and
- a DNA, positioned 3′ to the DNA encoding the signal peptide, encodes amino acids consisting of 79 to 883 of SEQ ID NO:2.
Dependencies are inferred from the claim text.