Self-containing Lactococcus strain
AssigneeActogenix N.V.
InventorLothar Steidler
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Abstract
The invention relates to a recombinant Lactococcus strain, with environmentally limited growth and viability. More particularly, it relates to a recombinant Lactococcus that can only survive in a medium, where well-defined medium compounds are present. A preferred embodiment is a Lactococcus that may only survive in a host organism, where such medium compounds are present, but cannot survive outside the host organism in the absence of such medium compounds.
Claims24
An isolated thymidylate synthase (thyA) mutant of a parent strain of Lactococcus species, said thyA mutant comprising:
- an inactive Lactococcus thymidylate synthase gene;
- and
- a gene encoding a heterologous therapeutic molecule;
- wherein said parent strain comprises SEQ ID NO:
- 3 and or SEQ ID NO:
- 5,
- wherein the thyA mutant expresses the heterologous therapeutic molecule.
An isolated thymidylate synthase (thyA) mutant of a parent strain of Lactococcus species, the thyA mutant comprising:
- an inactive Lactococcus thymidylate synthase gene and a gene encoding a heterologous therapeutic molecule, wherein the inactive Lactococcus thymidylate synthase gene has been inactivated by gene disruption;
- wherein the parent strain of Lactococcus species comprises:
- SEQ ID NO:
- 3 or SEQ ID NO:
- 5, and wherein the thyA mutant expresses the heterologous therapeutic molecule.
An isolated thymidylate synthase (thyA) mutant of a parent strain of Lactococcus species, wherein the thyA mutant lacks active Lactococcus thymidylate synthase gene and comprises a gene encoding a heterologous therapeutic molecule, wherein the thyA mutant expresses the heterologous therapeutic molecule, and wherein the thyA mutant is produced by a process comprising:
- providing a parent strain of Lactococcus species comprising a thymidylate synthase gene comprising wherein said Lactococcus thymidylate synthase gene comprises SEQ ID NO:
- 3 or SEQ ID NO:
- 5;
- and
- altering by gene disruption the Lactococcus thymidylate synthase gene of the parent strain so as to inactivate the thymidylate synthase encoded thereby.
Dependencies are inferred from the claim text.