Process for preparing a pentopyranosyl nucleic acid conjugate
AssigneeAventis Research & Technologies GmbH & Co. KG
InventorsChristian Miculka, Norbert Windhab, Tilmann Brandstetter, Gerhard Burdinski
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Abstract
The invention relates to a process for preparing a conjugate that includes a pentopyranosyl nucleic acid and a biomolecule. The process includes the steps of providing a pentopyranosyl nucleic acid having at least two pentopyranosyl nucleotide subunits that are covalently linked between carbon 4 and carbon 2 of their respective pentopyranosyl rings. The pentopyranosyl nucleic acid also has an electrophilic reactive group. A biomolecule having a nucleophilic reactive group is also provided. The electrophilic reactive group of the pentopyranosyl nucleic acid and the nucleophilic reactive group of the biomolecule are reacted to form a covalent bond.
Claims13
A process for preparing a conjugate comprising the steps of:
- providing a pentopyranosyl nucleic acid, wherein the pentopyranosyl nucleic acid has at least two pentopyranosyl nucleotide subunits that are covalently linked between carbon 4 and carbon 2 of their respective pentopyranosyl rings, and wherein the pentopyranosyl nucleic acid further comprises an electrophilic reactive group;
- providing a biomolecule comprising a nucleophilic reactive group;
- and
- reacting the electrophilic reactive group of the pentopyranosyl nucleic acid and the nucleophilic reactive group of the biomolecule to form a covalent bond.
Dependencies are inferred from the claim text.