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US 7,777,020 B2Granted
Nucleotide sequences derived from the env gene of HIV-1
AssigneesInstitut Pasteur, Institut National de la Sante et de la Recherche Medicale
InventorsMaurice Moncany, Luc Montagnier
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Abstract
This invention encompasses an env nucleic acid product produced by a process comprising providing a sample containing HIV-1 nucleic acid and amplifying the nucleic acid using primer pairs.
Claims9
An envelope (env) nucleic acid product produced by the process comprising:
- (a) providing a sample comprising an env gene of HIV-1 nucleic acid;
- and
- (b) amplifying a template comprising sequence of the env gene of the HIV-1 nucleic acid from the sample using at least one pair of purified polynucleotides chosen from:
- (1) a polynucleotide from each of group (i) and group (ii),
- wherein group (i) comprises nucleotides 6905-6930, 7055-7077, 7360-7384, 7832-7857, 8844-8869, 7629-7647, 8224-8242 of the env gene of HIV-1 Bru;
- and
- wherein group (ii) comprises nucleotides 6930-6905, 7384-7360, 7857-7832, 8869-8844, and 8242-8224 of a nucleic acid sequence complementary to the env gene of HIV-1 Bru;
- (2) a polynucleotide from each of group (iii) and group (iv),
- wherein group (iii) comprises nucleotides 6903-6928, 7053-7075, 7349-7373, 7821-7846, 8836-8861, 7612-7630, and 8213-8231 of the env gene of HIV-1 Mal;
- and
- wherein group (iv) comprises nucleotides 6928-6903, 7373-7349, 7846-7821, 8861-8836, 8231-8213 of a nucleic acid sequence complementary to the env gene of HIV-1 Mal;
- and
- (3) a polynucleotide from each of group (v) and group (vi),
- wherein group (v) comprises nucleotides 6860-6885, 7010-7032, 7306-7330, 7775-7800, 8787-8812, 7572-7590, and 8167-8185 of the env gene of HIV-1 Eli;
- and
- wherein group (vi) comprises nucleotides 6885-6860, 7330-7306, 7800-7775, 8812-8787, and 8185-8167 of a nucleic acid sequence complementary the env gene of HIV-1 Eli,
- wherein the amplification is carried out under conditions that result in specific amplification of the env nucleic acid product from HIV-1 nucleic acid.
14 more elements
A method for gene amplification of nucleic acids from HIV-1 viruses comprising:
- (a) providing a sample containing at least one HIV-1 nucleic acid;
- (b) amplifying the HIV-1 nucleic acid with at least two primers, wherein said first primer is complementary to a region of nucleotides of the nucleic acid of said genome, said second primer is complementary to a region of nucleotides of the strand of DNA complementary to said nucleic acid of said genome, wherein said regions of nucleotides are separated by about 100 to about 1100 base pairs when said complementary strands are hybridized to form one double-stranded nucleic acid, wherein:
- (1) the first and second primers comprise a polynucleotide from each of group (i) and group (ii), respectively,
- wherein group (i) comprises nucleotides 6905-6930, 7055-7077, 7360-7384, 7832-7857, 8844-8869, 7629-7647, 8224-8242 of the env gene of HIV-1 Bru;
- and
- wherein group (ii) comprises nucleotides 6630-6905, 7384-7360, 7857-7832, 8869-8844, and 8242-8224 of a nucleic acid sequence complementary to the env gene of HIV-1 Bru;
- (2) the first and second primers comprise a polynucleotide from each of group (iii) and group (iv), respectively,
- wherein group (iii) comprises nucleotides 6903-6928, 7053-7075, 7349-7373, 7821-7846, 8836-8861, 7612-7630, and 8213-8231 of the env gene of HIV-1 Mal;
- and
- wherein group (iv) comprises nucleotides 6928-6903, 7373-7349, 7846-7821, 8861-8836, 8231-8213 of a nucleic acid sequence complementary to the env gene of HIV-1 Mal;
- (3) the first and second primers comprise a polynucleotide from each of group (v) and group (vi), respectively,
- wherein group (v) comprises nucleotides 6860-6885, 7010-7032, 7306-7330, 7775-7800, 8787-8812, 7572-7590, and 8167-8185 of the env gene of HIV-1 Eli;
- and
- wherein group (vi) comprises nucleotides 6885-6860, 7330-7306, 7800-7775, 8812-8787, and 8185-8167 of a nucleic acid sequence complementary the env gene of HIV-1 Eli;
- c) and amplifying said nucleic acids under conditions that result in specific amplification of the env nucleic acid product from HIV-1 nucleic acid.
12 more elements
Dependencies are inferred from the claim text.