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US 7,776,553 B2Granted
Screening assays and methods
AssigneePresidents and Fellows of Harvard College
InventorsJ. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
Life of this patent
Estimating the term…
Abstract
Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.
Claims30
A method of screening a monoclonal antibody comprising:
- sealing a moldable slab to a substrate, said moldable slab comprising at least one microwell of less than 100 micrometers in diameter, each of said microwells comprising a single antibody-producing cell that secretes monoclonal antibodies in a volume of 10 nanoliters or less of fluid in said microwell allowing said antibody-producing cell to secrete said monoclonal antibodies in said volume;
- exposing said volume of said monoclonal antibodies to at least one defined antigen, and
- determining if said monoclonal antibodies binds to said antigen, wherein the steps of the method of screening the monoclonal antibody are performed in less than one day.
Dependencies are inferred from the claim text.