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US 7,772,012 B2Granted
Device for detecting the presence of hemoglobin in a biological sample
AssigneeQuest Diagnostics Investments Incorporated
InventorHoward Milne Chandler
Life of this patent
Estimating the term…
Abstract
A device and method for detecting the presence of hemoglobin in a biological sample, more particularly, the presence of blood in a fecal sample as an indicator of upper or lower gastrointestinal tract bleeding.
Claims10
A device for the detection of hemoglobin in a biological sample, comprising a carrier matrix which includes:
- (i) a sample application region for receipt of said biological sample;
- (ii) a first substrate region in liquid-conductive communication with, or combined with, the sample application region and having a pseudoperoxidase substrate loaded therein in a dried state;
- said pseudoperoxidase substrate comprising a peroxide or hydroperoxide reagent;
- and
- (iii) a first indicator region in liquid-conductive communication with, or combined with, the first substrate region and having an indicator applied thereto or impregnated therein, said indicator capable of producing a detectable response in the presence of heme and said pseudoperoxidase substrate;
- (iv) a second substrate region in liquid-conductive communication with the sample application region and having a detectable anti-globin immunointeractive molecule applied thereto or impregnated therein, said immunointeractive molecule forming a detectable globin-antiglobin complex in the presence of globin;
- and
- (v) a second indicator region in liquid-conductive communication with the second substrate region and having an anti-globin immunointeractive molecule immobilized therein, said immobilized immunointeractive molecule immobilizing said detectable globin-antiglobin complex.
5 more elements
A device for the detection of hemoglobin in a biological sample comprising:
- (i) a centrally disposed sample application region,
- (ii) a first laterally disposed test region, and
- (iii) a second laterally disposed test region,
- wherein each test region is in direct liquid-conductive communication with said sample application region, and
- wherein said first test region comprises a substrate region having a pseudoperoxidase substrate loaded therein in a dried state, and an indicator region having an indicator applied thereto or impregnated therein, said indicator capable of producing a detectable response in the presence of heme and said pseudoperoxidase substrate,
- wherein said second test region comprises a substrate region having a detectable antiglobin immunointeractive molecule applied thereto or impregnated therein, said immunointeractive molecule forming a detectable globin-antiglobin complex in the presence of globin;
- and a detection region having an antiglobin immunointeractive molecule immobilized therein, wherein said immunointeractive molecule is capable of immobilizing said detectable globin-antiglobin complex.
4 more elements
Dependencies are inferred from the claim text.