Salutaridinol 7-O-acetyltransferase and derivatives thereof
AssigneeDonald Danforth Plant Science Center
InventorsToni M. Kutchan, Meinhart H. Zenk, Torsten Grothe
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Abstract
This invention provides a salutaridinol 7-O-acetyltransferase protein, a salutaridinol 7-O-acetyltransferase gene, and a sequence which is complementary thereto. This invention further provides a method for the production of thebaine comprising the steps of (i) contacting in vitro a protein having salutaridinol 7-O-acetyltransferase activity with salutaridinol and acetyl coenzyme A at pH 8 to 9, and (ii) recovering the thebaine thus produced.
Claims10
An isolated nucleic acid comprising a nucleotide sequence that encodes a protein having at least 85% identity with the amino acid sequence of SEQ ID NO:
- 14 over a length of at least 400 amino acids and has salutaridinol 7-O-acetyltransferase activity, wherein said protein has a DFGWG motif (SEQ ID NO:20), a H163-XXX-D167 motif, a conserved serine (S33), and a conserved cysteine (C152).
A cell transformed or transfected by a nucleic acid comprising a nucleotide sequence that encodes a protein having at least 85% identity with the amino acid sequence of SEQ ID NO:
- 14 over a length of at least 400 amino acids and has salutaridinol 7-O-acetyltransferase activity, wherein said protein has a DFGWG motif (SEQ ID NO:
- 20), a H163-XXX-D167 motif, a conserved serine (S33) and a conserved cysteine (C152).
A method for producing a protein having salutaridinol 7-O-acetyltransferase activity, said method comprising
- i) transforming or transfecting a cell with a nucleic acid comprising a nucleotide sequence that encodes a protein having salutaridinol 7-O-acetyltransferase activity and at least 85% identity with the amino acid sequence of SEQ ID NO:
- 14 over a length of at least 400 amino acids, wherein said protein has a DFGWG motif (SEQ ID NO:
- 20), a H163-XXX-D167 motif, a conserved serine (S33), and a conserved cysteine (C152), in conditions permitting the expression of the protein having salutaridinol 7-O-acetyltransferase activity,
- ii) propagating said cells, and
- iii) recovering the thus-produced protein having salutaridinol 7-O-acetyltransferase activity.
Dependencies are inferred from the claim text.