Bone delivery conjugates and method of using same to target proteins to bone
AssigneeEnobia Pharma Inc.
InventorsPhilippe Crine, Guy Boileau, Isabelle Lemire, Thomas P. Loisel
Life of this patent
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Abstract
A bone delivery conjugate having a structure selected from the group consisting of: A) X-Dn-Y-protein-Z; and B) Z-protein-Y-Dn-X, wherein X is absent or is an amino acid sequence of at least one amino acid; Y is absent or is an amino acid sequence of at least one amino acid; Z is absent or is an amino acid sequence of at least one amino acid; and Dn is a poly aspartate wherein n=10 to 16. Compositions comprising same and methods of use thereof.
Claims8
A bone delivery conjugate comprising the structure:
- Z-protein-Y-Dn-X,
- wherein X is absent or is an amino acid sequence of at least one amino acid;
- Y is absent or is an amino acid sequence of at least one amino acid;
- Z is absent or is an amino acid sequence of at least one amino acid;
- Dn is a poly aspartate wherein n=10 to 16;
- and
- wherein said protein is a secreted soluble form of an alkaline phosphatase (sALP) having an amino acid sequence encoded by the sequence as set forth in SEQ ID NO:
- 5.
A method of delivering ALP to bone tissue of a mammal in need thereof comprising administering to said mammal an effective amount of a bone delivery conjugate comprising the structure:
- Z-protein-Y-Dn-X,
- wherein X is absent or is an amino acid sequence of at least one amino acid;
- Y is absent or is an amino acid sequence of at least one amino acid;
- Z is absent or is an amino acid sequence of at least one amino acid;
- Dn is a poly aspartate wherein n=10 to 16;
- and
- wherein said protein is a secreted soluble form of an alkaline phosphatase (sALP) having an amino acid sequence encoded by the sequence as set forth in SEQ ID NO:
- 5.
A method of treating a condition or disease related to a bone defect characterized by a lack of or an insufficient amount of functional alkaline phosphatase comprising administering to a mammal in need thereof a conjugate comprising the structure:
- Z-protein-Y-Dn-X,
- wherein X is absent or is an amino acid sequence of at least one amino acid;
- Y is absent or is an amino acid sequence of at least one amino acid;
- Z is absent or is an amino acid sequence of at least one amino acid;
- Dn is a poly aspartate wherein n=10 to 16;
- and
- wherein said protein is a secreted soluble form of an alkaline phosphatase (sALP) having an amino acid sequence encoded by the sequence as set forth in SEQ ID NO:
- 5;
- and
- and wherein the conjugate is in a pharmaceutically acceptable carrier.
Dependencies are inferred from the claim text.