Sushi peptide multimer
AssigneeNational University of Singapore
InventorsJeak Ling Ding, Bow Ho
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Abstract
Endotoxin, also known as lipopolysaccharides (LPS), is the major mediator of septic shock due to Gram-negative bacterial infection. Chemically synthesized S3 peptide, derived from Sushi3 domain of Factor C, which is the endotoxin-sensitive serine protease of the limulus coagulation cascade, binds and neutralizes LPS activity. Fluorescent tagged-S3 is shown to detect LPS-containing bacteria. For large-scale production of S3 and to mimic other pathogen-recognizing molecules, tandem multimers of the S3 gene were constructed and expressed in E. coli. Tetramer of S3 for example is shown to display an enhanced inhibitory effect on LPS-induced activities. An affinity matrix based on tetramer of S3 is also shown to be particularly efficient at removing LPS.
Claims17
A polypeptide comprising more than one S3 peptides comprising the amino acid sequence set forth in SEQ ID No:
- 1, wherein at least two of the S3 peptides are separated by a linking sequence cleavable by a protease.
A polypeptide comprising more than one S3 peptides comprising the amino acid sequence set forth in SEQ ID No:
- 1, wherein at least two of the S3 peptides are separated by a linking sequence cleavable by acid digestion.
A polypeptide comprising the amino acid sequence set forth in SEQ ID NO:9, having a linking sequence cleavable by a protease.
Dependencies are inferred from the claim text.