US 7,759,114 B2 · Sensor chips

Abstract

A method for selectively orienting molecules on a surface of a solid support. The method includes the steps of: (a) attaching a linker molecule to the surface of the solid support, the linker molecule including a head group that is capable of binding to the solid support, and a tail group that is capable of chelating to a metal ion; (b) subsequently treating the solid support with a solution containing the metal ion; (c) attaching a metal ion chelating tag to the molecules to form tagged molecules; and (d) capturing the tagged molecules on the solid support by contacting it with the tagged molecules to form a monolayer of molecules on the surface of the solid support in which a majority of the molecules are held in the same orientation with respect to the surface. The invention also provides a sensor chip formed using the methods of the invention.

Claims12

Dependent claims

A biosensor comprising:

a solid support having a surface;
a plurality of linkers attached to the surface, wherein each linker includes a head group that is bound to the surface, and a multidentate macrocylic tail group comprising 1-acetato-4-benzyl-1,4,7-triazacyclononane chelated to a metal ion;
and
a monolayer of molecules immobilised to the solid support by the plurality of linkers and having a binding affinity for an analyte, wherein each molecule of the monolayer contains a metal ion chelating tag that coordinates with the metal ion on a tail group of a linker, thereby holding a majority of the molecules of the monolayer in the same orientation with respect to the surface of the solid support.

1 more element

Dependencies are inferred from the claim text.

Sensor chips

Life of this patent

Sensor chips

Life of this patent

Abstract

Claims12