Fabrication of tissue lamina using microfabricated two-dimensional molds

An apparatus capable of supplementing or replacing at least one organ function comprising multiple layers of tissue lamina stacked and fastened together,

• wherein each layer of tissue lamina comprises:
• (a) a first polymer scaffold configured with a plurality of branched microchannels and having endothelial cells seeded within the branched microchannels to form a vasculature system;
• (b) a second polymer scaffold having parenchymal cells within the scaffold to form a parenchymal tissue;
• and
• (c) a semi-permeable membrane disposed between the first and second polymer scaffolds, the semi-permeable membrane configured to allow exchange of oxygen, nutrients and waste between fluid circulating in the vasculature system of the first polymer scaffold and the parenchymal tissue present in the second polymer scaffold;
• wherein the first polymer scaffold and the second polymer scaffold are fastened together about the semi-permeable membrane.