# US 7,753,955 B2 · Methods and composition for soft tissue feature reconstruction

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- Number: US 7,753,955 B2
- Document ID: US7753955B2
- Status: Granted
- Kind: B2
- Application number: 10/580,559
- Filed: 2004-12-10
- Published: 2007-11-22
- Granted: 2010-07-13
- Earliest claimed: 2003-12-10
- Assignees: Cellular Bioengineering, Inc.
- Inventors: Hank C. K. Wuh
- Web page: https://patentgrep.com/patent/US7753955B2

## CPC

- A61L 27/38: {containing added animal cells (organs or tissue containing native cells A61L27/36)}
- A61L 27/303: {Carbon}
- A61L 27/60: Materials for use in artificial skin
- C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)}
- C12N 5/0075: {using microcarriers}
- C12N 2533/30: Synthetic polymers
- C12N 2533/50: Proteins
- C12N 2533/54: Collagen; Gelatin
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

This present invention describes methods and compositions useful for the reconstruction of various soft tissue features such as lips, areola, and many other features by taking a mold of the skin feature to be replaced, such as the areola, prior to surgical resection, re-creating the size and shape of the soft tissue feature, for example, the nipple and areola, and making a polymer or biopolymer scaffold that is biocompatible, has the ability to allow the epithelization of the skin cells over the polymer, the capability of cell integration into the body of the scaffold, as well as the capability of infiltration of surrounding nerve fibers into the substance of the scaffold, so that the patient may have the benefit of a reconstructed soft tissue feature that not only has the same size and shape and appearance as the native tissue, but also has functional sensation.

## Claims

### Claim 1

A biocompatible substrate for the cosmetic reconstruction of a mammalian soft tissue feature or region comprising:
a) a scaffold comprising a polymer, biopolymer or combination thereof in a thin sheet, microparticle or semi-solid block form;
b) embedding or incorporating into the scaffold; during its synthesis attachment or growth promoting reagents consisting essentially of one or more of the following: laminin, fibronectin, RGDS, bFGF conjugated with polycarbophyll, EGF conjugated with polycarbophyll, and heparin sulfate; and
c) wherein the scaffold is molded into the shape of a mammalian skin epithelial feature or region.

### Claim 2 (depends on 1)

The biocompatible substrate of claim 1, wherein the soft tissue features to be reconstructed can be any soft tissue feature such as a human ear, areola, nose, lip, genitalia, fingertip, and nail bed.

### Claim 3 (depends on 1)

The biocompatible substrate of claim 1, wherein the scaffold is selected from the group comprising:
a) natural polymers including but not limited to collagen, gelatin, hyaluronate, fibrin and alginate;
b) synthetic polymers including but not limited to polyacrylic acid and derivatives, polyethylene oxide and copolymers, polyvinyl alcohol, polyphosphazene, polypeptides; PNIPAAM/gelatin; NIPAAM; and
c) further compositions comprising mixtures of natural polymers in a) and synthetic polymers in b).