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US 7,750,128 B2Granted
Modified Fc molecules
AssigneeAmgen Inc.
InventorsColin Gegg, Fei Xiong, Karen C. Sitney
Life of this patent
Estimating the term…
Abstract
The present invention concerns compositions of matter, for example, but not limited to, modified antibodies, in which one or more biologically active peptides are incorporated into a loop region of a non-terminal domain of an immunoglobulin Fc domain.
Claims18
A composition of matter of the formula
- (X1)a—F1 (X2)b
- and multimers thereof, wherein:
- F1 is an IgG2 Fc domain comprising SEQ ID NO:
- 606 modified so that it comprises at least one X3 inserted into or replacing all or part of a sequence selected from SEQ ID NOS:
- 621, 622, 624, 632, 636, 639, 640, 642, 644, and 646 within a loop region of the IgG2 Fc domain, said loop region being in a non-terminal domain of the Fc domain;
- X1 and X2 are each independently selected from -(L1)c-P1, -(L1)c-P1-(L2)d-P2, -(L1)c-P1-(L2)d-P2-(L3)e-P3, and -(L1)c-P1-(L2)d-P2-(L3)e-P3-(L4)fP4;
- X3 is independently selected from -(L5)c-P5, -(L5)c-P5-(L6)d-P6, -(L5)c-P5-(L6)d-P6-(L7)e-P7, and -(L5)c-P5-(L6)d-P6-(L7)e-P7-(L8)f-P8;
- P1, P2, P3, and P4 are each independently sequences of pharmacologically active polypeptides or pharmacologically active peptides;
- P5, P6, P7, and P8 are each independently sequences of pharmacologically active peptides;
- L1, L2, L3, L4, L5, L6, L7, and L8 are each independently linkers;
- and
- a, b, c, d, e, and f are each independently 0 or 1.
9 more elements
A modified antibody, comprising an Fc domain, F1, wherein:
- F1 is an IgG2 Fc domain comprising SEQ ID NO:
- 606 modified so that it comprises at least one X3 inserted into or replacing all or part of a sequence selected from SEQ ID NOS:
- 621, 622, 624, 632, 636, 639, 640, 642, 644, and 646 within a loop region of the IgG2 Fc domain, said loop region being in a non-terminal domain of the Fc domain, wherein:
- X3 is independently selected from -(L5)c-P5, -(L5)c-P5-(L6)d-P6, -(L5)c-P5-(L6)d-P6-(L7)e-P7, and -(L5)c-P5-(L6)d-P6-(L7)e-P7-(L8)f-P8;
- P5, P6, P7, and P8 are each independently sequences of pharmacologically active peptides;
- L5, L6, L7, and L8 are each independently linkers;
- and
- c, d, e, and f are each independently 0 or 1.
5 more elements
Dependencies are inferred from the claim text.