Non-competitive immunoassay for small analytes
AssigneeValtion Teknillinen Tutkimuskeskus
InventorsTimo Pulli, Matti Hoyhtya, Kristiina Takkinen, Hans Soderlund
Life of this patent
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Abstract
A non-competitive immunoassay for small analytes, wherein the analyte is reacted with two binding partners. The first binding partner binds to the analyte to form a complex between the first binding partner and the analyte, and the second binding partner binds to the complex formed by the first binding partner and the analyte. The resulting complex formed between the analyte and the binding partners is detected. The binding partners are proteins, such as antibodies including antibody fragments.
Claims13
A non-competitive immunoassay for detecting a small analyte, said assay comprising:
- reacting a sample containing said analyte with a reagent pair comprising a first binding partner that binds to said analyte, and a second binding partner that binds to the complex of said analyte and said first binding partner, wherein said second binding partner is obtained from a non-immunized source which is a naive display recombinant binding partner library by selecting a binding partner that binds to said complex of the analyte and first binding partner, and determining the binding of the second binding partner, thus indicating the presence of the analyte in the sample, wherein the analyte has a molecular weight of less than 5000.
A non-competitive immunoassay for detecting a small analyte, said assay comprising:
- reacting a sample containing said analyte with a reagent pair comprising a first binding partner that binds to said analyte, and a second binding partner that binds to the complex of said analyte and said first binding partner, wherein said second binding partner is obtained from a non-immunized source which is a naive display recombinant binding partner library by selecting a binding partner that binds to said complex of the analyte and first binding partner, and determining the binding of the second binding partner, thus indicating the presence of the analyte in the sample, wherein the analyte has a molecular weight of less than 5000, wherein the second binding partner comprises a ligand binding portion of K11 scFv comprising SEQ ID NO 5.
A non-competitive immunoassay for detecting a small analyte, said assay comprising:
- reacting a sample containing said analyte with a reagent pair comprising a first binding partner that binds to said analyte, and a second binding partner that binds to the complex of said analyte and said first binding partner, wherein said second binding partner is obtained from a non-immunized source which is a naive display recombinant binding partner library by selecting a binding partner that binds to said complex of the analyte and first binding partner, and determining the binding of the second binding partner, thus indicating the presence of the analyte in the sample, wherein the analyte has a molecular weight of less than 5000, wherein the first binding partner comprises a ligand-binding portion of M1 Fab comprising SEQ ID NO 1 and SEQ ID NO 2, or of M2 Fab comprising SEQ ID NO 3 and SEQ ID NO 4.
Dependencies are inferred from the claim text.